富硒长双歧杆菌优势菌株的高通量选育

运用96孔深孔板微型化培养和酶标仪快速检测，建立富硒长双歧杆菌（Bifidobacterium longum）优势菌株的高通量筛选方法。通过高通量筛选获得的富硒长双歧杆菌优势菌株XBL-30，具有很好的硒蛋白转化能力。在7 L罐中添加一定浓度的亚硒酸钠，优势菌株XBL-30的硒蛋白转化率从原始菌株的60%提高至85.3%，菌株多次传代后，硒蛋白转化率均能稳定在85%。该研究建立的高通量筛选方法不仅简单，快速，成本低，而且对其他微生物菌株的高通量筛选具有一定的参考价值。

Screening of dominant strains of selenium-rich Bifidobacterium longum by high-throughput screening method

A high-throughput screening(HTS)method for screening dominate strains of selenium-rich Bifidobacterium lonfidum was developed through 96-deep MTPs and microplate reader.A dominate strain of selenium-rich B.lonfidum XBL-30 was successfully screened,which had good selenoprotein conversion ability.When certain sodium selenite was added to 7 L tank,the selenoprotein conversion rate of the dominate strain XBL-30 increased from the original strain of 60% to 85.3%.The selenoprotein conversion rate could be stabilized at 85%after repeated passage.The high-throughput screening method established in the study was not only simple,fast and low cost,but also had certain reference value for large-scale screening of other microbial strains.