基于高通量测序技术巴彦淖尔市酸粥中细菌多样性分析

采用Illumina MiSeq高通量测序技术对采集自内蒙古自治区巴彦淖尔市的10份酸粥样品中的细菌多样性进行解析，同时基于细菌属水平对其进行围绕中心点的划分（PAM），并基于PICRUSt和BugBase软件对细菌的基因功能及表型进行预测。结果表明，所有酸粥样品中的细菌隶属于9个细菌门的55个属，样品间细菌多样性和丰度存在明显差异，但优势细菌门均为硬壁菌门（Firmicutes）和变形菌门（Proteobacteria），优势细菌属均为乳杆菌属（Lactobacillus）和醋杆菌属（Acetobacter）。所有酸粥样品可分为两个聚类，聚类I以醋杆菌属（Acetobacter ）为主，聚类II以乳杆菌属（Lactobacillus）为主；两个聚类中细菌对于碳水化合物的利用和细菌的生长繁殖等存在显著差异（P＜0.05），隶属于聚类I的酸粥样品中，其细菌的代谢和繁殖更加迅速。

Bacterial diversity in sour porridge from Bayannur based on high-throughput sequencing

The bacterial diversity of 10 sour porridge samples collected from Bayannur City, Inner Mongolia Autonomous regionwas analyzed by Illumina MiSeq high-throughput sequencing technique. Partitioning around medoid (PAM) analysis was carried out based on the level of bacterial genera, and the gene function and phenotype of bacteria were predicted based on PICRUSt and BugBase software. The results showed that the bacteria in all sour porridge samples belonged to 55 genera of 9 phyla, and there were significant differences in bacterial diversity and abundance among all sour porridge samples, but the dominant bacteria in sour porridge were Firmicutes and Proteobacteria, which were all Lactobacillus and Acetobacter. All sour porridge samples could be divided into two clusters, cluster I was mainly Acetobacter, and cluster II was mainly Lactobacillus. There were significant differences in the utilization of carbohydrates and the growth and reproduction of bacteria in the two clusters (P＜0.05), and the metabolism and reproduction of bacteria in the sour porridge samples belonging to cluster I were more rapid.