葛根发酵乳发酵条件优化

以从酸奶中筛选的高产胞外多糖（EPS）的乳酸菌为出发菌株，用葛根和脱脂乳进行乳酸菌的发酵，通过单因素试验及响应面试验对葛根发酵乳发酵条件进行优化。结果表明，最优的发酵条件为发酵温度39 ℃，葡萄糖添加量1.4%，发酵时间20 h。在此优化条件下，葛根发酵乳总黄酮含量为1.91 mg/mL，EPS含量为0.155 mg/mL。

Optimization of fermentation conditions of fermented pueraria-milk

Using the lactic acid bacteria with high yield extracellular polysaccharides(EPS)screened from yogurt as the original strain,pueraria and skim milk were fermented by lactic acid bacteria.The fermentation conditions of pueraria and skimmed milk were optimized by single factor experiments and response surface methods.Results indicated that the optimal fermentation condition was fermentation temperature 39℃,glucose addition 1.4%,and time 20 h.Under the condition,the total flavonoid content of pueraria yogurt was 1.91 mg/ml,and the EPS content was 0.155 mg/ml.