| 市售生鲜湿面中腐败菌的分离与鉴定 |
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| 引用本文： | 王欢,李刚凤,夏欣欣,马莉敏,吴兴泉.市售生鲜湿面中腐败菌的分离与鉴定[J].中国酿造,2020,39(9):148. |
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| 作者姓名： | 王欢 李刚凤 夏欣欣 马莉敏 吴兴泉 |
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| 作者单位： | （1.河南工业大学 生物工程学院，河南 郑州 450000；2.铜仁学院 材料与化学工程学院，贵州 铜仁 554300） |
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| 基金项目： | 贵州省教育厅大学生创新创业训练中心项目（2016SJDCZX001） |
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| 摘 要： | 采用涂布平板法从生鲜湿面中分离腐败菌，通过形态观察及分子生物学技术对分离腐败菌进行菌种鉴定。结果表明，从生鲜湿面中分离得到8株典型菌株，包括3株霉菌和5株细菌。经鉴定，3株霉菌（A1，B1，C1）分别为草酸靑霉（Penicillium oxalicum）、黄曲霉（Aspergillus flavus）、黑曲霉（Aspergillus niger）；5株细菌（S1～S5）分别为3株蜡状芽孢杆菌（Bacillus cereus）、1株肠炎沙门氏菌（Salmonella enterica）、1株副芽孢杆菌（Bacillus paramycoides）。
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| 关 键 词： | 生鲜湿面 腐败菌 分离 鉴定 |
Isolation and identification of spoilage bacteria in commercial fresh wet noodles |
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| WANG Huan,LI Gangfeng,XIA Xinxin,MA Limin,WU Xingquan.Isolation and identification of spoilage bacteria in commercial fresh wet noodles[J].China Brewing,2020,39(9):148. |
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| Authors: | WANG Huan LI Gangfeng XIA Xinxin MA Limin WU Xingquan |
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| Affiliation: | (1.College of Bioengineering, Henan University of Technology, Zhengzhou 450000, China; 2.College of Material and Chemical Engineering, Tongren University, Tongren 554300, China) |
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| Abstract: | Spoilage bacteria were isolated from fresh wet noodles by spread-plates method, and identified by morphological observation and molecular biology techniques. The results showed that 8 typical strains were isolated from fresh wet noodles, including 3 molds (A1, B1, C1) and 5 bacteria (S1-S5). The 3 strains of molds were identified as Penicillium oxalicum, Aspergillus flavus and Aspergillus niger, respectively. The 5 strains of bacteria were identified as 3 strains of Bacillus cereuses, 1 strain of Salmonella enteric and 1 strain of Bacillus paramycoides. |
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| Keywords: | fresh wet noodles spoilage bacteria isolation identification |
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