基于MiSeq测序技术石花酒大曲中微生物多样性解析

该研究采用Illumina MiSeq高通量测序技术对襄阳市石花酒大曲中微生物多样性进行分析。结果表明，4个样品中优势细菌门（＞0.1%）为变形杆菌门（Proteobacteria）、厚壁菌门（Firmicutes）和放线菌门（Actinobacteria），且均为核心菌门；优势细菌属（＞1%）为假单胞菌属（Pseudomonas）、芽孢杆菌属（Bacillus）、乳杆菌属（Lactobacillus）、链霉菌属（Streptomyces）、魏斯氏菌属（Weissella）、明串珠菌属（Leuconostoc）和Kroppenstedtia。优势真菌门（＞0.1%）为子囊菌门（Ascomycota）、担子菌门（Basidiomycota）和Mucoromycota，其中子囊菌门为核心菌门；优势真菌属（＞1%）为复膜孢酵母属（Saccharomycopsis）、红曲菌属（Monascus）、热子囊菌属（Thermoascus）、青霉菌属（Penicillium）、曲霉属（Aspergillus）和Leiothecium。样品中假单胞菌属和曲霉属呈现显著正相关（P＜0.05）。综上，4个石花酒大曲样品中共有大量的核心菌群，且样品中细菌与真菌有一定的相关性。

Analysis of microbial diversity in Shihua Baijiu Daqu based on MiSeq technology

In this study, the microbial diversity in Shihua Baijiu (Chinese liquor) Daqu in Xiangyang was analyzed by Illumina MiSeq high-throughput sequencing technology. The results showed that the dominant phyla of bacteria (＞0.1%) in 4 samples were Proteobacteria, Firmicutes and Actinobacteria, which were core bacteria phyla. The dominant bacteria genera (＞1%) were Pseudomonas, Bacillus, Lactobacillus, Streptomyces, Weissella, Leuconostoc and Kroppedtia. The dominant phyla of fungi (＞0.1%) were Ascomycota, Basidiomycota and Muscomycota, among them, the Ascomycota was core fungus phylum. The dominant genera of fungi (＞1%) were Saccharomycopsis, Monascus, Thermoascus, Penicillium, Aspergillus and Leiothecium. There was a significant positive correlation between Pseudomonas and Aspergillus in the sample (P＜0.05). In conclusion, there were a large number of core microflora in 4 Shihua Baijiu Daqu samples and some correlations between bacteria and fungi.