| 中心组合优化重组毕赤酵母生产S-腺苷甲硫氨酸用发酵培养基 |
| |
| 引用本文： | 朱闪,储炬,胡晓清,庄英萍,张嗣良,袁中一. 中心组合优化重组毕赤酵母生产S-腺苷甲硫氨酸用发酵培养基[J]. 高技术通讯, 2006, 16(2): 181-185 |
| |
| 作者姓名： | 朱闪 储炬 胡晓清 庄英萍 张嗣良 袁中一 |
| |
| 作者单位： | 1. 华东理工大学生物反应器工程国家重点实验室,国家生化工程技术研究中心,上海,200237
2. 中国科学院上海生命科学研究院生物化学和细胞生物学研究所,上海,200031 |
| |
| 摘 要： | 利用统计学实验设计方法优化了重组毕赤酵母生产S-腺苷甲硫氨酸(SAM)的发酵培养基.采用中心组合设计和响应面分析对诱导相培养基中硫酸铵、甲醇和L-甲硫氨酸三个关键因素的浓度进行了优化,使SAM在全合成培养基上的摇瓶产量提高了60%,达到1.9g/L,并考察了胞内SAM合成酶活性的变化与SAM积累的相关性.
|
| 关 键 词： | 响应面分析 S-腺苷甲硫氨酸 培养基优化 重组毕赤酵母 SAM合成酶 |
| 收稿时间： | 2005-04-14 |
| 修稿时间： | 2005-04-14 |
Medium optimization for S-adenosyl-L-methionine production by recombinant Pichia pastoris using statistics-based experimental design |
| |
| Zhu Shan,Chu Ju,Hu Xiaoqing,Zhuang Yingping,Zhang Siliang,Yuan Zhongyi. Medium optimization for S-adenosyl-L-methionine production by recombinant Pichia pastoris using statistics-based experimental design[J]. High Technology Letters, 2006, 16(2): 181-185 |
| |
| Authors: | Zhu Shan Chu Ju Hu Xiaoqing Zhuang Yingping Zhang Siliang Yuan Zhongyi |
| |
| Affiliation: | 1.State Key Lab of Bioreactor Engineering, National Engineering Center for Biotechnology, East China University of Science and Technology, Shanghai 200237;2.Institute of Biochemistry and Cell Biology, Shanghai Institutes for Life Science, Chinese Academy of Sciences, Shanghai 200031 |
| |
| Abstract: | Statistically-based experimental designs were used to optimize a chemically defined medium for the S-adenosyl-L-me-thionine(SAM) production by recombinant Pichia pastoris. The concentrations of ammonium sulfate, methanol and L-me-thionine were optimized using central composite design and response surface analysis. SAM production was increased by 60% when the optimization strategy was applied. Furthermore, the relationship between the amount of SAM synthetase and the accumulation of SAM was studied. |
| |
| Keywords: | response surface analysis S-adenosyl-L-methionine(SAM) medium optimization recombinant Pichia pastoris SAM synthetase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
