牙鲆淋巴囊肿病毒核酸疫苗的安全性研究

对牙鲆淋巴囊肿病毒核酸疫苗进行了安全性鉴定.试验采用PCR方法,通过检测该疫苗是否与牙鲆染色体整合,以及疫苗中的卡那霉素抗性基因是否对牙鲆体内和环境中抗性菌数量及种类产生影响,来确认疫苗的安全性.结果表明,该疫苗不与牙鲆染色体发生整合,也未对环境中的抗性菌数量和种类产生影响,表明本实验用核酸疫苗对于受免牙鲆和环境都是安全的.

The safety assessment of DNA vaccine encoding lymphocystis disease virus

Lymphocystis Disease Virus(LCDV-cn)disease is the first virosis which is prevalent cosmically in the cultured Japanese flounder Paralichthys olivaceus. The paper presents the safety validation results. The validation was based on whether the DNA vaccine integrates with Paralichthys olivaceus chromosomes and whether the Kanamycine-resistant gene of the vaccine affects the resistant bacteria amounts and varieties inside as well as outside of the paralichthys olivaceus, both were determined by PCR measurement. The results showed that the DNA vaccine did not integrate with paralichthys olivaceus chromosomes; neither did it affect the amounts and varieties of the resistant bacteria in the surroundings. The DNA vaccine was proved to be safe for both the paralichthys olivaceus and the environment.