可溶性Fas与VEGF及TIMP-1表达在乳腺癌淋巴结转移中的相互关系

目的通过对乳腺癌可溶性Fas浓度与血管内皮生长因子(VEGF)及金属蛋白酶抑制物(TIMP-1)表达之间关系的分析,探讨患者血清中sFas浓度改变对乳腺癌细胞生长、浸润和转移的影响。方法通过S-P免疫组化法和ELISA法,分别检测乳腺癌细胞VEGF和TIMP-1表达及血清sFas浓度,结合VEGF、TIMP-1与乳腺癌临床病理参数,分析浸润、淋巴结转移乳腺癌中VEGF、TIMP-1表达失衡与血清sFas浓度之间的关系。结果①VEGF阳性表达与乳腺癌淋巴结转移呈显著相关,VEGF阳性的肿瘤有淋巴结转移率为56%,明显高于VEGF阳性的肿瘤无淋巴结转移率(7%)。随着乳腺癌TNM分期和组织学分级的进展,VEGF阳性表达率逐渐增加,呈正相关。TIMP-1阳性表达与肿瘤淋巴结转移明显相关。随着TNM分期和组织学分级的进展,TIMP-1阳性表达率逐渐降低,呈负相关。②VEGF阳性表达而TIMP-1阴性表达组发生乳腺癌浸润和淋巴结转移率及血清sFas浓度最高,VEGF阴性表达而TIMP-1阳性表达组发生淋巴结转移率及血清sFas浓度最低,两组比较差异有极显著意义。结论sFas与VEGF及TIMP-1表达之间存在着反馈机理以及相互激活的密切关系。sFas浓度可以间接地反映基质金属蛋白酶(MMPs)和VEGF的表达情况,sFas在乳腺癌中的浓度变化可以反映乳腺癌的生长、局部浸润和转移。

Relationship between Soluble Fas Concentration and Expression of Vascular Endothelial Growth Factor(VEGF) and Tissue Inhibitor of Metalloproteinase-1 ( TIMP-1 ) in Metabasis of Breast Cancer in Lymph Node

Objective To analyze the relationship between soluble Fas(sFas) concentration and the expressions of vascular endothelial growth factor(VEGF) and tissue inhibitor of metalloproteinase-1(TIMP-1) in breast cancer and explore the influence of sFas concentration on the growth,infiltration and metabasis of breast cancer.Methods Determine the VEGF,TIMP-1 and serum sFas concentration of patients with breast cancer by S-P immunohistochemical method and ELISA respectively,and analyze the relationship among the unbalanced expression of VEGF and TIMP-1 and the serum sFas concentration in breast cancer with infiltration and metabasis in lymph node by the combination of determination results with the pathological parameters of breast cancer in clinic.Results The expression of VEGF was significantly related to the metabasis of breast cancer in lymph node.Of the VEGF-positive tumors,56% showed metabasis in lymph node and only 7% showed no metabasis in lymph node.The expression rate of VEGF was positively related to the progress of TNM phases and histological stages of breast cancer.Significant relationship was also observed between the expression of TIMP-1 and the metabasis of breast cancer in lymph node.The expression rate of TIMP-1 was negatively related to the progress of TNM phases and histological stages of breast cancer. The tumors positive for VEGF but negative for TIMP-1 showed the highest infiltration rate and metabasis rate in lymph node as well as the highest serum sFas concentration.However,the tumors negative for VEGF but positive for TIMP-1 showed the lowest metabasis rate in lymph node and the lowest serum sFas concentration.Conclusion Feedback mechanism existed among sFas,VEGF and TIMP-1,which could activate each other and showed close relationship.sFas concentration reflected the expressions of matrix metalloproteinase(MMPs) and VEGF indirectly,and its change reflected the growth,local infiltration and metabasis of breast cancer.