Optimization of macroporous 3-D silk fibroin scaffolds by salt-leaching procedure in organic solvent-free conditions |
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Authors: | Xinghua Zhang Chuanbao Cao Xilan Ma Yanan Li |
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Affiliation: | (1) Research Center of Material Science, Beijing Institute of Technology, Beijing, 100081, China; |
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Abstract: | A novel all-aqueous process is described to form three-dimensional porous silk fibroin (SF) scaffolds, which not only avoided
the use of organic solvents or harsh chemicals, but also can form scaffolds with various sizes and in large quantities. The
scaffolds show a rough surface on the pores and the pores are highly interconnected. The porosity of the scaffolds, which
varied between a large range (67.6~99.3%), can be controlled by the SF concentrations and the salt/fibroin ratio. The results
of measurements indicated that this novel process can improve and enforce the transformation in SF structure from a random
coil to a β-sheet. Swelling studies showed that the scaffold has excellent properties of hydrophilicity. The cell culture
experiments demonstrated that the scaffolds facilitated the human osteosarcoma cells attachment and proliferation in vitro. |
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