Anti-complementary activity of flavonoids from Gnaphalium affine D. Don |
| |
Authors: | Zhongxin Xi Wansheng Chen Zhijun Wu Yan Wang Peiyuan Zeng Guijun Zhao Xia Li Lianna Sun |
| |
Affiliation: | 1. School of Pharmacy, Second Military Medical University, Guohe Road 325#, Shanghai 200433, People’s Republic of China;2. Department of Pharmacy, Fujian University of Traditional Chinese Medicine, Hutuo Road 1#, Fuzhou 350108, People’s Republic of China;3. Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Fengyang Road 415#, Shanghai 200003, People’s Republic of China |
| |
Abstract: | Gnaphalium affine D. Don, is used as a vegetable as well as a folk medicine in China for its anti-inflammatory, antitussive, and expectorant activities. Phytochemical investigation of the ethyl acetate (EtOAc) fraction of this plant led to isolation of three new acylated flavonol glycosides, apigenin 4′-O-β-d-(6″-E-caffeoyl)-glucopyranoside, luteolin 4′-O-β-d-(6″-E-caffeoyl)-glucopyranoside, and quercetin 4′-O-β-d-(6″-E-caffeoyl)-glucopyranoside, together with 24 known compounds. The structures of these compounds were determined by spectroscopic methods. All compounds were evaluated for their anti-complementary activity on the classical pathway of the complement system in vitro, and luteolin 4′-O-β-d-(6″-E-caffeoyl)-glucopyranoside exhibited highest anti-complementary activity with an IC50 value of 0.045 ± 0.005 mg/ml. This is the first report of anti-complementary activity of G. affine D. Don, which displays the initial evidence that this plant is a potent complement inhibitor. |
| |
Keywords: | Gnaphalium affine D. Don Anti-complement Acylated flavonol glycosides Apigenin 4&prime -O-β-d-(6&Prime -E-caffeoyl)-glucopyranoside Luteolin 4&prime -O-β-d-(6&Prime -E-caffeoyl)-glucopyranoside Quercetin 4&prime -O-β-d-(6&Prime -E-caffeoyl)-glucopyranoside |
本文献已被 ScienceDirect 等数据库收录! |
|