Isolation of some active compounds from Origanum vulgare L. ssp. vulgare and determination of their genotoxic potentials |
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Authors: | Medine Gulluce Mehmet Karadayi Zuhal Guvenalp Hilal Ozbek Tulin Arasoglu Ozlem Baris |
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Affiliation: | 1. Department of Biology, Science Faculty, Atatürk University, 25240 Erzurum, Turkey;2. Department of Pharmacognosy, Pharmacy Faculty, Atatürk University, 25240 Erzurum, Turkey;3. Istanbul Regional Hygiene Institute, Ministry of Health Turkey, 34020 Istanbul, Turkey |
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Abstract: | Origanum vulgare L. (oregano) is a flavoring herb widely used around the world. In the present study, crude extract of O. vulgare L. (oregano) and its petroleum ether, chloroform, ethyl acetate, n-butanol, water fractions were prepared in order to isolate and investigate antimutagenic compounds from the active extract through the bacterial genotoxicity assay guided fractionation procedures. The methanol extract and its n-butanol fraction showed significant antimutagenic activity. In the end of sub-fractionation process of the n-butanol extract, luteolin 7-O-glucuronide and luteolin 7-O-xyloside were isolated. These compounds showed significant antimutagenic activity against 9-Aminoacridine and N-Methyl-N′-nitro-N-nitrosoguanidine mutagenicity. The inhibition rates ranged from 22.1% (luteolin 7-O-xyloside: Salmonella typhimurium TA1537 – 0.4 mM/plate) to 67.8% (luteolin 7-O-glucuronide: S. typhimurium TA1537 – 0.8 μM/plate). In conclusion, the results revealed that luteolin 7-O-glucuronide and luteolin 7-O-xyloside are two of the antimutagenic compounds of O. vulgare L. ssp. vulgare. |
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Keywords: | Origanum vulgare L. ssp. vulgare Luteolin Antimutagenicity Ames test Escherichia coli WP2 test |
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