Quantitative analysis of phenolic components and glycoalkaloids from 20 potato clones and in vitro evaluation of antioxidant,cholesterol uptake,and neuroprotective activities |
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Authors: | Xiuhong Ji Lucas Rivers Zosia Zielinski Min Xu Erinn MacDougall Jancy Stephen Shuocheng Zhang Yanwen Wang Robert G Chapman Paul Keddy George S Robertson Christopher W Kirby Jean Embleton Kraig Worrall Agnes Murphy David De Koeyer Helen Tai Lilian Yu Edward Charter Junzeng Zhang |
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Affiliation: | 1. Institute for Nutrisciences and Health, National Research Council of Canada, 550 University Avenue, Charlottetown, PE, Canada C1A 4P3;2. Departments of Psychiatry and Pharmacology, Faculty of Medicine, Dalhousie University, 5850 College Street, Sir Charles Tupper Medical Building, Halifax, NS, Canada B3H 1X5;3. Crops and Livestock Research Centre, Agriculture and Agri-Food Canada, 550 University Avenue, Charlottetown, PE, Canada C1A 4P3;4. Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, Fredericton, NB, Canada E3B 4Z7;5. Bio|Food|Tech, 101 Belvedere Avenue, Charlottetown, PE, Canada C1A 7N8 |
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Abstract: | Potato (Solanum tuberosum L.) is one of the most important food crops in the world and provides essential nutrients. With an aim to develop potato varieties for functional food or nutraceutial applications, we have conducted metabolomic profiling, total phenolics, chlorogenic acid, anthocyanins, and glycoalkaloids analyses on 20 selected potato clones within the Canadian potato breeding program of Agriculture and Agri-Food Canada. Pigmented potatoes in general contain higher levels of phenolic components, including chlorogenic acid and anthocyanins. Levels of phenolics were retained with granulation processing of pigmented potato tubers, but glycoalkaloids were significantly reduced with granulation. The pigmented potatoes also have higher antioxidant activity reaching up to 35% of that for berries, measured as their potency in scavenging DPPH radicals. Extracts of the 20 potato clones (peel, tuber, and granule) were also evaluated for in vitro effects on liver LDL cholesterol uptake and protection of cortical neurons from cell death caused by oxygen glucose deprivation (OGD). These potato extracts in general showed mild activity in enhancing LDL cholesterol uptake in liver HepG2 cells, and also protected cortical neurons against OGD induced cell death, with extracts from granules of six of the potato clones showing significant neuroprotective effects. The bioactive components are not dependent on pigmentation of potato clones. These novel bioactivities identified in potatoes warrant in-depth investigations in the future. Taken together, our results provide further evidence for the enhanced health beneficial components in potato. |
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Keywords: | Solanum tuberosum Pigmented potato Phenolics Anthocyanins Glycoalkaloids Antioxidant Cholesterol uptake Neuroprotection |
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