Non-toxic Salvia sclareoides Brot. extracts as a source of functional food ingredients: Phenolic profile,antioxidant activity and prion binding properties |
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Authors: | Amélia P Rauter Catarina Dias Alice Martins Isabel Branco Nuno R Neng José M Nogueira Margarida Goulart Filipa VM Silva Jorge Justino Clare Trevitt Jon P Waltho |
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Affiliation: | 1. Universidade de Lisboa, Faculdade de Ciências, Departamento de Química e Bioquímica/Centro de Química e Bioquímica, Edifício C8, 5° Piso, Campo Grande, 1749-016 Lisboa, Portugal;2. Escola Superior Agrária de Santarém, Instituto Politécnico de Santarém, Quinta do Galinheiro, 2001-904 Santarém, Portugal;3. Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK;4. Faculty of Life Sciences and Manchester Interdisciplinary Biocentre, The University of Manchester, Manchester M1 7DN, UK |
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Abstract: | Salvia sclareoides is an aromatic herb native to Portugal, of which phenolic content (Folin–Ciocalteau method), chemical profile (HPLC/DAD), antioxidant activity (DPPH, β-carotene/linoleic acid assays), acute toxicity (MTT method, adapted for non-adherent cells), genotoxicity (short-term chromosomal aberration assay) and prion binding properties were evaluated in the acetone, water, ethanol, methanol and n-butanol extracts. The latter presented the highest phenolic content and antioxidant activity (DPPH assay), and was the single one with the flavonoids (+)-catechin, kaempferol O-glucoside and quercetin. Vanillic acid was the major component of all extracts but gallic, gentisic, caffeic, syringic, coumaric and ferulic acids were also found in some extracts. Only the n-butanol extract had components binding to the cellular form of human prion protein detected by NMR which showed specificity for two regions of the folded domain and for the unstructured N-terminal region. Extracts were not cytotoxic nor genotoxic, reinforcing the potential of S. sclareoides for nutraceutical purposes. |
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Keywords: | Salvia sclareoides Phenolic profile Antioxidant activity Cytotoxicity Genotoxicity Prion binding |
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