联氨基姜黄素对乳腺癌细胞MCF-7增殖和凋亡的影响及其机制

目的探讨联氨基姜黄素对人乳腺癌细胞MCF-7增殖和凋亡的影响及其机制。方法采用MTT法检测联氨基姜黄素对MCF-7细胞的抗增殖效应,Hochest33258染色观察细胞形态学变化,流式细胞术分析细胞的周期分布和凋亡情况,Western blot检测MCF-7细胞中Bcl-2、Bax、Cyclin D1和Survivin蛋白的表达变化。结果联氨基姜黄素可抑制MCF-7细胞的增殖,IC50为2.56μmol/L,而姜黄素的IC50为21.22μmol/L;联氨基姜黄素染色24 h后,MCF-7细胞出现核荧光强度增强、颗粒状荧光等凋亡特征;凋亡细胞比率明显增加,并可阻滞细胞周期于G1期,且呈一定的剂量依赖性;联氨基姜黄素可使MCF-7细胞中Bcl-2、Cyclin D1、Survivin蛋白表达水平明显降低,而Bax表达增加。结论联氨基姜黄素具有抑制MCF-7细胞增殖、促进凋亡、阻滞细胞周期于G1期的作用,其机制可能与Bcl-2、Bax、Cyclin D1、Survivin蛋白的表达改变有关。

Effect of hydrazinocurcumin on proliferation and apoptosis of breast cancer MCF-7 cells and relevant mechanism

Objective To investigate the effect of hydrazinocurcumin(HC)on proliferation and apoptosis of human breast cancer MCF-7 cells as well as the relevant mechanism.Methods The proliferation-inhibiting effect of HC on MCF-7 cells was meas ured by MTT assay.The morphological changes of MCF-7 cells was observed by Hochest33258 staining.The cell cycle distribution and apoptosis of MCF-7 cells were determined by flow cytometry.The expressions of Bcl-2,Bax,Cyclin D1 and survivin were determined by Western blot.Results HC inhibited the proliferation of MCF-7 cells,with an IC50 of 2.56 μmol/L,while that of curcumin was 21.22 μmol/L.Some characteristics of apoptosis,such as increased fluorescent intensity in nuclei and granular fluorescence,were observed in MCF-7 cells 24 h after treatment with HC,while the proportion of apoptotic cells increased significantly,while G1 arrest appeared.The expression levels of Bcl-2,Bax,Cyclin D1 and Survivin in MCF-7 cells after treatment with HC decreased significantly,while that of Bax increased.Conclusion HC inhibited the proliferation and promoted the apoptosis of MCF-7 cells,and resulted in G1 arrest,by a mechanism which might be related to the changes of expressions of Bcl-2,Bax,Cyclin D1 and surviving.