响应曲面法优化超声—微波协同提取百合渣中总皂苷工艺的研究

以宜兴百合淀粉生产过程中产生的副产物百合渣为原料,采用超声-微波协同提取工艺提取其中的总皂苷,通过乙醇浓度、液料比、微波功率、提取时间各单因素实验确定各因素对总皂苷得率的影响。在单因素实验的基础上,根据Box-Behnken实验原理,采用了四因素三水平的响应面分析法,以总皂苷的得率作为响应值,根据所得实验结果进行回归分析,优选出提取的最佳工艺为:乙醇浓度77%,液料比11∶1(mL/g),微波功率503W,提取时间139s,在此最佳条件下,测得总皂苷的得率为6.59‰。该工艺运用了超声与微波的协同效应,集取了超声与微波二者优势,具有提取时间短、效率高的优点。

Optimization of ultrasound-microwave synergistic extraction of total saponins from lily residue by response surface methodology

The residue of the by-products in the starch production process of Yixing lily was analyzed as the extracted material to study the optimum procedures of the ultrasound-microwave synergy extraction method.Effects of thanol concentration,liquid-solid ratio,microwave power and extraction time on yield of total saponins were determined. Based on the results of single factor test,as well as Box-Behnken experiment principle,the four factors and three levels response surface method(RSM) test was designed and the yield of total saponins was determined as response value. The optimum extracting parameters to achieve the highest total saponins yield of 6.59‰ was obtained as follows:ethanol concentration(v/v) of 77%,liquid to solid ratio of 11∶1(mL/g),microwave power of 503W,and extraction time 139s. The ultrasound-microwave synergy extraction technique combined with the advantages of microwave and ultrasonic was a new method of higher efficiency,lower temperature and less time consumption.