牡蛎酶解产物改善小鼠学习记忆能力的初步研究

探讨了牡蛎酶解产物对小鼠空间学习记忆能力的影响。选用中性蛋白酶、动物蛋白酶和复合酶分别水解牡蛎蛋白得到EHO1、EHO2和EHO3,分析其基本营养成分和分子量分布;采用Morris水迷宫行为学方法考察EHO对小鼠学习记忆的改善能力。结果显示,EHO含有丰富的肽、糖原、牛磺酸,分子量在主要集中在1300～10000u之间;定位航行测试显示,相对于空白组,EHO干预后小鼠的潜伏期都明显的缩短,空间搜索策略的比例明显提高,EHO3组效果最好,潜伏期(16.77±7.74)s(p<0.05),空间搜索的比例为85.71%;而空间探索实验中,给药组小鼠的穿越次数、中央活动时间和目标象限路程比都有不同程度的增加。实验结果表明一定剂量的EHO能够提高小鼠的空间学习和记忆能力。 

Preliminary research on the function of enzymatic hydrolysis of oyster to improve the learning and memory in mice

Investigation of the effect of enzymatic hydrolysis of oyster(EHO)on learning and memory ability.The contents of major components and the molecular weight distribution of the EHO prepared respectively by adding neutrase,proteolytic enzymes and mixed enzymes were test,then the learning and memory ability were assessed using the Morris water maze(MWM)with one control group.The results showed that the EHO was rich in peptide,glycogen and taurine,and the molecular weights were from 1300～10000u.MWM test indicated that the proportion of spatial strategy of the treated groups were higher and the escape latency were significantly shorter compared to the control group.EHO3 group was the best one,the escape latency was(16.77±7.74)s(p<0.05)and the proportion of spatial strategy reached 85.71%.All the three EHO groups had more number of crossings,longer time in the center and longer distance in the target quadrant than that of control group in the spatial probe test.These findings suggested that certain amount of EHO could improve the learning and memory ability of mice.