| 硼酸对L-阿拉伯糖异构酶催化合成D-塔格糖的影响 |
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| 引用本文： | 李晓卉,缪铭,沐万孟,江波,张涛.硼酸对L-阿拉伯糖异构酶催化合成D-塔格糖的影响[J].食品工业科技,2012,33(11):315-317. |
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| 作者姓名： | 李晓卉 缪铭 沐万孟 江波 张涛 |
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| 作者单位： | 江南大学食品科学与技术国家重点实验室,江苏无锡,214122 |
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| 基金项目： | 江苏省社会发展科技支撑项目(BE2010626,BE2011622和BE2011766) |
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| 摘 要： | 基于硼酸与构型不同的单糖的络合能力不同,探讨了添加硼酸对L-阿拉伯糖异构酶催化D-半乳糖异构化为D-塔格糖的酶反应体系的影响,研究了pH、温度、硼酸加入量、加酶量等条件对硼酸作用的影响。结果表明:当反应条件为pH9.0,温度65℃,硼酸与底物的摩尔比1∶2,加酶量为0.39U/mL时,90g/L的底物D-半乳糖的转化率为50%,而不加硼酸时转化率为27%,表明硼酸对反应有很好的促进作用。
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| 关 键 词： | 硼酸 半乳糖 塔格糖 络合 |
Production of D-tagatose using L-arabinose ismoerase through addition of boric acid |
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| LI Xiao-hui,MIAO Ming,MU Wan-meng,JIANG Bo,ZHANG Tao.Production of D-tagatose using L-arabinose ismoerase through addition of boric acid[J].Science and Technology of Food Industry,2012,33(11):315-317. |
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| Authors: | LI Xiao-hui MIAO Ming MU Wan-meng JIANG Bo ZHANG Tao |
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| Affiliation: | (State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China) |
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| Abstract: | Based on different affinities of boric acid to D-galactose and D-tagatose,an L-arabinose ismoerase mutant enzyme was used to catalyze the isomerization of D-galactose to D-tagatose with boric acid.The effect of pH,temperature,molar ratio of boric acid and the enzyme addition was studied.The results showed the optimum conditions for production of D-galactose were pH 9.0,65℃,1∶2 molar ratio of boric acid to D-galactose,the amounts of enzyme was 0.39U/mL.Under this condition,in 16h,the convertion was 50% when the concentration of D-galactose was 90g/L with boric acid,while 27% in the Tris-HCl buffer system without borate,which showed that boric acid had good impact on the production of D-tagatose. |
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| Keywords: | boric acid galactose tagatose complexing |
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