| 大孔树脂对节节草总黄酮吸附分离特性研究 |
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| 引用本文： | 张俊生,朱士龙,李俊,陈莉华,张文龙.大孔树脂对节节草总黄酮吸附分离特性研究[J].食品工业科技,2012,33(13):236-239. |
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| 作者姓名： | 张俊生 朱士龙 李俊 陈莉华 张文龙 |
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| 作者单位： | 吉首大学化学化工学院,湖南吉首,416000 |
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| 基金项目： | 科技部科技型中小企业技术创新基金资助项目(10C26214302421,11C26414305373);吉首大学校级科研计划项目(11JDY051) |
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| 摘 要： | 考察了D-101大孔吸附树脂对节节草黄酮类物质的吸附及解吸特性,对黄酮类物质的吸附平衡和吸附动力学进行了深入探讨。结果表明,D-101树脂对节节草总黄酮的吸附等温线符合Langmuir方程,吸附动力学规律可用二级速率方程表示。D-101大孔吸附树脂纯化节节草黄酮类物质的适宜工艺参数为:黄酮质量浓度为2mg/mL、pH2.1、吸附液料比20:1、静置时间60min、50mL95%乙醇溶液为洗脱剂时,洗脱率达到99.01%。纯化后,黄酮质量分数从10.54%提高到60.01%。
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| 关 键 词： | 节节草 黄酮 大孔树脂 纯化 |
Study on adsorption and separation characteristics of flavonoids from Equisetum ramosissimum Desf.with macroporous resin |
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| ZHANG Jun-sheng,ZHU Shi-long,LI Jun,CHEN Li-hua ,ZHANG Wen-long.Study on adsorption and separation characteristics of flavonoids from Equisetum ramosissimum Desf.with macroporous resin[J].Science and Technology of Food Industry,2012,33(13):236-239. |
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| Authors: | ZHANG Jun-sheng ZHU Shi-long LI Jun CHEN Li-hua ZHANG Wen-long |
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| Affiliation: | (College of Chemistry and Chemical Engineering,Jishou University,Jishou 416000,China) |
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| Abstract: | The application of D-101 macroporous resin for the adsorption and desorption of total flavonoids from Equisetum ramosissimum Desf.was investigated.The adsorption equilibrium and adsorption kinetics were discussed in detail.The data obtained from kinetic and isotherm experiments could be well described using pseudo-second-order rate model and Langmuir isotherm equation respectively.The practicable technical parameters for adsorption/desorption of total flavonoids by D-101 resin were as follows:2.0mg/mL flavonoids solution concentration,pH2.1,amount(desorption solvent volume/D-101 resin weight ratio) 20:1,standing time 60min and eluting with 50mL 95% ethanol,under which the desorption rate could reach 99.01%.The purity of total flavonoids was increased from 10.54% to 60.01%. |
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| Keywords: | Equisetum ramosissimum Desf flavonoid D-101 macroporous resin purification |
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