超滤制备海带岩藻聚糖的工艺研究

本文以海带为原料,研究应用超滤技术提取岩藻聚糖的工艺技术参数,研究结果表明:冷浸提法可以防止岩藻聚糖的降解;pH低于2.5时80%以上的褐藻酸会被除去;超滤过程中,压力在0.1～0.3MPa范围内随着压力的升高膜通量升高;浸提液经稀释0～2倍下膜通量升高,多糖得率变化不大。研究结论:在4℃下以料液比1:30浸提5h所得浸提液,调pH至2.0以除去褐藻酸,补蒸馏水至3000mL,采用50ku超滤膜组件在0.2MPa下超滤纯化岩藻聚糖,经醇沉冷冻干燥制得高纯度海带岩藻聚糖干品,其得率为2.32%,总糖含量为38.24%,硫酸基团含量达到21.49%。 

Application of ultrafiltration on the extraction of fucoidan from kelp polysaccharide

This paper studied that it was feasible to apply membrane separation technology to extract fucoidan from kelp polysaccharide.Also,the best technical parameters had been fixed on.It demonstrated that extraction in cold water could protect fucoidan from degradation.More than 80% of alginate could be removed at the condition of pH≤2.5.With the increasing of operating pressure,membrane flux would increase during the ultrafiltration process.After diluting the extract 0～2times,membrane flux increased,but the extracting ratio almost didn’t changed.The results showed that the best extracting condition were:temperature 4℃,rate of material to liquid 1:30,extract 5 hours.Alginate removed under the condition of pH=2.0 50ku of ultrafiltration membrane was used to purify fucoidan under the pressure of 0.2MPa.The permeated liquid was precipitated by ethanol and dried by vacuum-freeze drying.The yield of fucoidan was 2.32%.The compositions of total sugar and sulphate were 38.24% and 21.49%.