有机碳源对单针藻细胞生长、油脂积累和光合作用的影响

研究不同有机碳源对单针藻(Monoraphidium sp.)细胞生长,油脂积累和光合作用的影响,探讨其细胞生长和油脂积累的最佳碳源浓度。结果表明单针藻具有利用有机碳源进行混合营养生长的能力,葡萄糖、蔗糖对其细胞生长、总脂含量和光合放氧速率具有明显的促进作用,甘氨酸能够促进细胞生长,但总脂含量下降,乙酸钠则表现为抑制作用;在BG-11培养基中加入5g/L葡萄糖后,细胞生物量、总脂产量和光合放氧速率分别为7.8、3.2g/L和240.3μmolO2·(mg·chla)-1·h-1,是同等光合自养条件下的5.6、8.0和1.3倍;单针藻细胞生长、总脂积累的最佳葡萄糖添加浓度为10g/L。

Effect of organic carbon sources on growth rate,lipid productivity and photosynthesis of Monoraphidium sp.cells

The effect of different organic sources and the best concentration of organic source for growth rate,total lipid content and photosynthesis of Monoraphidium sp.cells were investigated.Results showed that the addition of glucose and sucrose could promote the growth rate,total lipid content and photosynthesis of Monoraphidium sp.cells,the addition of glycine could promote the growth rate of Monoraphidium sp.cells,but cut down the total lipid content.Besides,the cell growth was obstructed when sodium acetate was added in.It indicated that Monoraphidium sp.cells had the ability of utilizing the organic carbon sources to grow mixotrophically.In BG-11 medium with glucose concentration of 5g/L,the dry weight,total lipid productivities and photosynthesis of Monoraphidium sp.were 7.8,3.2g/L and 240.3umolO2·(mg·chla)-1·h-1,respectively,which were 5.6,8.0,1.3 folds of that obtained under autophototrophic condition.The best concentration of organic source for growth rate and total lipid content of Monoraphidium sp.cells was 10g/L.