灰毡毛忍冬芽提取物的抑菌活性及其镇痛抗炎解热作用的研究

目的：观察灰毡毛忍冬芽提取物的抑菌活性和镇痛抗炎解热作用。方法：采用平板纸片法和微孔稀释法观察其抑菌活性并考察其抑菌稳定性;以小鼠福尔马林致痛及醋酸扭体实验观察其镇痛作用;以小鼠耳廓肿胀模型和角叉菜胶致大鼠足肿胀模型观察其抗炎作用;用酵母致动物发热法观察其解热作用。结果：灰毡毛忍冬芽提取物对金黄色葡萄球菌、大肠杆菌、八叠微球菌、绿脓杆菌、枯草芽孢杆菌和伤寒杆菌均有抑菌效果,其MIC分别为375、187.5、93.75、187.5、187.5、187.5mg·mL-1,MBC分别为1500、750、375、1500、750、1500mg·mL-1。提取物在pH1～3的酸性条件下抑菌效果最好,但高温长时间处理和紫外光长时间照射均可降低其抑菌活性;与纯水组相比,生药含量为15g·kg-1的灰毡毛忍冬芽提取物组能显著提高小鼠福尔马林实验中的痛域,减少醋酸所致小鼠的扭体次数,对二甲苯所致小鼠耳廓的肿胀度抑制率达36.62%,可以抑制角叉菜胶所致的大鼠足肿胀,并能有效对抗酵母所致大鼠的体温升高。结论：灰毡毛忍冬芽提取物在体外对金黄色葡萄球菌、大肠杆菌、八叠微球菌、绿脓杆菌、枯草芽孢杆菌和伤寒杆菌均有良好的抑菌效果,并具有一定的镇痛、抗炎、解热作用。

Study on the bacteriostatic activity,anti-inflammation,analgesic and antipyretic effects of extract from Lonicera bud

Objective:To study the bacteriostatic activity,anti-inflammation,analgesic and antipyretic effects of extract from Lonicera bud.Methods:The antibacterial activity of the extract against six common bacteria was assessed by measuring its MIC and MBC by the disk diffusion method and the microporous dilution method.The analgesic effects of Lonicera bud were assayed by the formaldehyde test and the writhing test in mice.The anti-inflammation effects of Lonicera bud were tested by the mouse’s both ears edemata caused by dimethylbenzene and rat’s foot swelling caused by carrageenan.The antipyretic effects of the Lonicera bud extract was tested by using the animal heating method caused by yeas.Results:Lonicera tea extracts in vitro on the 6 kinds of bacteria has significant bacteriostatic effect.The respectively of MIC was 375,187.5,93.75,187.5,187.5,187.5mg·mL-1.MBC was 1500,750,375,1500,750,1500mg·mL-1.The extracts exhibited excellent antimicrobial activities under acidic conditions(pH1～3),whereas both high-temperature treatment and long-time UV illumination attenuated the antimicrobial activities.Extctra from Lonicera bud(15g·kg-1)significantly increased the pain threshold of mice in the formaldehyde test compared to the control,and reduced the writhing number,and obviously inhibited edemata of the mouse’s ears caused by dimethybenzene by 36.62% and that of the rat’s tarsus caused by para-carrageenin as well as reduced the elevated body temperature induced by yeast.Conclusion:Lonicera tea extracts had the bacteriostatic effect on the 6 kinds of bacteria in vitro.Extract from Lonicera bud had a anti-inflammatory and analgesic antipyretic effect to some extent.