鞣花酸和石榴皮多酚提取物抗氧化活性的比较

对鞣花酸提取物(EA)和石榴皮多酚提取物(PE)的抗氧化活性进行比较研究。通过HPLC法检测EA和PE中鞣花酸的含量,Folin-酚法检测PE中总多酚含量;采用H2O2处理HepG2细胞建立细胞氧化应激损伤模型,MTT法比较不同浓度的EA和PE对HepG2细胞氧化应激损伤的保护作用;采用DPPH法、·OH清除法检测PE和EA体外抗氧化作用。结果表明:与EA相比,PE中鞣花酸含量很低,但PE和EA均以剂量依赖的方式提高氧化损伤的HepG2细胞生存率,有显著的保护作用,且在同一的浓度下,PE的保护作用显著高于EA。PE和EA对DPPH法、·OH清除法的清除率均呈剂量依赖性增强。

Comparative study of antioxidant activities of ellagic acid and pomegranate peel extract

The comparative study was conducted on the antioxidant activities of ellagic acid extract(EA) and pomegranate peel extract(PE).The content of ellagic acid in PE and EA were detected by HPLC,and the content of total polyphenol in PE by folin-phenol.Oxidative stress experimental model of cells was established with HepG2 cell treated by H2O2.Then the MTT was employed to determinate the protective effects of various levels of PE and EA for HepG2 cell against oxidative stress injury.DPPH method and ·OH scavenging method were used to estimate the free radicles elimination ability in vitro of PE and EA.The result showed that the content of ellagic acid was much lower in PE than that in EA,but both PE and EA,in a dose-reliant way,increased the survival rate of HepG2 cells which were under oxidative stress injury-they demonstrated remarkable protective effect.At the same time,the protective effect of PE was markedly better than that of EA.The elimination rate of both PE and EA upon DPPH method and ·OH scavenging method was also in a dose-reliant fashion.