胀果甘草悬浮细胞中黄酮类化合物HPLC分析

用HPLC法建立野生胀果甘草与悬浮培养的胀果甘草细胞中黄酮类化合物的HPLC指纹图谱,并采用LC-MS技术推测HPLC共有峰中主要指纹峰的化学结构。采用Agilent EclipseXDB-C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈:0.1%冰醋酸,梯度洗脱,流速1.0 mL/min,检测波长310 nm。Agilent1100LC/MSD液质联用系统,ESI源。在设定的色谱条件下,分别得到的野生甘草与甘草细胞中黄酮类化合物指纹图谱,用中国药典相似度评价系统软件进行评价相似度为75.2%,根据LC-MS结果推断了HPLC共有峰中2个主要指纹峰分别为甘草苷和甘草素。结果表明野生甘草中绝大部分黄酮类化合物在甘草细胞中均有合成,使利用细胞悬浮培养生产的甘草黄酮类化合物成为可行的途径。

Studies on the HPLC Fingerprint of Flavonoids in Suspension Culture Cell of Glycyrrhiza Inflata Bat

HPLC method was applied to determine of the fingerprint of flavonoids in wild and cell suspension cultured of Glycyrrhiza inflata Bat,and the chemical constituents of mainly fingerprints among the common HPLC peaks were analyzed by high performance liquid chromatography/tandem mass spectrometry(LC-MS).Agilent Eclipse XDB-C18 column(4.6 mm×250 mm,5 μm) was used,the mobile phase was consisted of acetonitrile-0.2 % acetic acid with gradient elution,at a flow rate of 1.0 mL/min.The detection wavelength was set at 310 nm,and the system of LC-MS was from Agilent 1100LC/MSD with electrospray ionization,under the selected condition,the HPLC fingerprint chromatograms of wild and cultured cell of Glycyrrhiza inflata were established and compared with professional software,the similarity was 75.2 %.The two mainly fingerprints among common HPLC peaks were identified as liquiritin and liquiritigenin by the ESI-MS.The results shown that most of flavonoids in radix was synthesized in the suspension cultured cell.