正交试验法优化香柏(变种)中总黄酮提取工艺

研究提取香柏总黄酮的最佳工艺条件。以总黄酮的得率为考察指标,溶剂回流作为提取手段,利用单因素和正交试验,考察提取温度、提取时间、乙醇溶液体积分数、液固比以及提取次数对总黄酮得率的影响。最优工艺条件为:在80℃水浴条件下,提取时间90 min、乙醇溶液体积分数60%、液固比50∶1(mL/g)、提取次数3次。在最优工艺条件下总黄酮的提取率可达7.68 g/100 g。该方法操作方便、准确可靠,可以用于工业化生产。

Optimization of Total Flavonoids Extraction from Sabina pingii(Cheng ex Ferre) Cheng et W.T. Wang var. wilsonii(Rehd.) Cheng et L.K. Fu Using the Orthogonal Test

Determine the optimal condition of extraction for total flavones in Sabina pingii(cheng ex Ferre) Cheng et W.T.Wang var.wilsonii(Rehd.) Cheng et L.K.Fu.Extractions of Reflux was investigated.During the extracting process,five main factors including,extraction temperature,processing time,concentration of extracting solution,liquid-solid ratio,extraction times were studied by using single factor analysis method.Based on the single-factor experiment,the best conditions of extracting total flavones were optimized by orthogonal test.The optimums of reflux extraction were: extracted in 80 ℃ water bath,ethanol concentration 60 %,processing time 90 min;extraction times 3,liquid-solid ratio 50∶1(mL/g);under the above conditions,the total flavones yield 7.68 %.Those extraction methods were simple,rapid and applicable for the extraction of total flavones in Sabina pingii(cheng ex Ferre) Cheng et W.T.Wang var.wilsonii(Rehd.) Cheng et L.K.Fu.