反相液相色谱法测定膏状香精中邻苯二甲酸酯类

采用反相液相色谱法研究了膏状香精中邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二正丁基酯（DBP）、邻苯二甲酸丁基苄基酯（BBP）4种邻苯二甲酸酯的萃取条件。利用正交实验对影响萃取总量的3个主要因素（正己烷用量、超声时间、水浴温度）进行了优化,确定了萃取的最佳条件:正己烷用量10mL,超声时间25min,水浴温度15℃。结果表明,该分析条件下,样品猪骨膏、牛肉膏的加标回收率为90%⁹4%。用乙腈-水等梯度洗脱（75∶25）,4种PAEs分离较好,在0.5²0mg/L之间,色谱峰面积与4种邻苯二甲酸酯的浓度呈现良好的线性关系。 

Determination of phthalate plasticizers in cream essence by reverse phase liquid chromatography

Four phthalate acid esters in cream essence,such as dimethyl phthalate(DMP),diethyl phthalate(DEP),dibutyl phthalate(DBP) and benzyl butyl phthalate(BBP),were determined by reverse phase liquid chromatography. The conditions for extraction were researched. Orthogonal design experiment was carried out to optimize the 3 dominant factors(hexane volume,ultrasonic time,water temperature) affecting extraction recovery. The optimum conditions for solid phase extraction were determined as follows:hexane volume 10mL,ultrasonic time 25min,water temperature 15℃. Results showed that the recoveries of the four phthalates were 90%~94%.There was a good linear relationship between peak area and concentration of 4 phthalate esters in range of 0.5~20mg/L.