丁香多酚细胞抗氧化活性的研究

目的：研究丁香多酚对由H2O2引起的人肝细胞RBL氧化损伤的保护作用。方法：采用H2O2诱导建立细胞氧化损伤模型,通过测定细胞存活率、细胞抗氧化酶活性、丙二醛等指标,分析探讨丁香多酚对细胞损伤的保护作用。结果：结果表明,100μmol/L H2O2孵育24h可显著诱导RBL细胞损伤,使细胞存活力下降到24.64%,细胞经不同浓度的丁香多酚（0.1、0.5、2、10mg/L）与H2O2共孵育后,特别是10mg/L丁香多酚可使细胞存活率达到59.18%。丁香多酚可通过提高SOD、CAT、GSH-Px酶活性,降低MDA含量,促进受损的RBL细胞修复。结论：丁香多酚对H2O2诱导氧化损伤的RBL细胞具有显著的保护作用,可作为食源性抗氧化剂。

Study on the antioxidant capacity of clove polyphenols in cell

Objective:A study was conducted to investigate the protective effects of clove polyphenols extracts on RBL caused by the damage of H2O2 and its protective mechanism.Method:Oxidative damage was induced by H2O2,and cell viability was measured by the MTT assay.The influence of clove polyphenols extracts on H2O2 induced RBL injury was assessed by measuring the superoxide dismutase(SOD),catalase(CAT),and glutathione peroxidase(GSH-Px)activities and the malondialdehyde(MDA)content.Result:The results demonstrated that RBL cells were damaged by incubation with 100μmol/L H2O2 for 24h,and the viability of RBL cells reduced to 24.64%.The addition of clove polyphenols extracts(0.1,0.5,2 and 10mg/L)into the RBL cell suspensions prior to the exposure to 100μmol/L of H2O2 resulted in a greater survival rate of the cells.In particular,the cell viability reached 59.18% after treating with 10mg/L clove polyphenols extracts.Moreover,at elevated concentrations,clove polyphenols extracts exhibited increased repairing capability for injured RBL as well as increased protection of SOD,CAT and GSH-PX while reduced MDA formation.Conclusion:The clove polyphenols extracts possessed protective effects on RBL cell injuries induced by H2O2,and this may be related to the antioxidative activity of clove polyphenols.