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| 利用线粒体DNA Cyt b基因PCR-RFLP分析方法鉴别羊肉和鸭肉 | |
| 引用本文： | 冯海永,刘丑生,何建文,安添午,韩建林.利用线粒体DNA Cyt b基因PCR-RFLP分析方法鉴别羊肉和鸭肉[J].食品工业科技,2012,33(13):319-321. |
| 作者姓名： | 冯海永 刘丑生 何建文 安添午 韩建林 |
| 作者单位： | 1. 农业部种畜品质监督检验测试中心,北京,100193 2. 甘肃农业职业技术学院,甘肃兰州,730020 3. 四川省草原科学研究院,四川成都,611731 4. 中国农业科学院一国际家畜研究所畜禽牧草遗传资源联合实验室,中国农业科学院北京畜牧兽医研究所,北京100193 |
| 基金项目： | “十一五”国家科技支撑计划项目(2007BAD52B05);CAAS-ILRI联合研究项目;中国农业科学院一级岗位杰出人才基金 |
| 摘 要： | 建立了一种利用线粒体DNA(mtDNA) Cyt b基因PCR-RFLP分析来鉴别羊肉和鸭肉的方法。采用一对通用引物扩增绵羊、山羊和鸭的mtDNACytb基因,并对扩增产物用DNA限制性内切酶Bsu36I和SpeI进行酶切,电泳分析酶切产物的变化。结果表明通用引物可扩增羊和鸭472bp的PCR产物,经两种内切酶酶切后,绵羊、山羊和鸭的PCR产物分别被切为大小不同的片段,其中绵羊和山羊被SpeI切为213bp和259bp,而鸭则被Bsu36I切为95bp和377bp。利用PCR-RFLP分析mtDNA Cyt b基因的方法操作简单,是一种快速鉴别羊肉和鸭肉的可靠方法。 |
| 关 键 词： | PCR-RFLP 物种鉴别 线粒体DNACytb基因 |
Identification of mutton from duck meat by PCR-RFLP analysis of mitochondrial DNA Cyt b gene |
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| FENG Hai-yong,LIU Chou-sheng,HE Jian-wen,AN Tian-wu,HAN Jian-lin.Identification of mutton from duck meat by PCR-RFLP analysis of mitochondrial DNA Cyt b gene[J].Science and Technology of Food Industry,2012,33(13):319-321. | |
| Authors: | FENG Hai-yong LIU Chou-sheng HE Jian-wen AN Tian-wu HAN Jian-lin |
| Affiliation: | 4,*(1.Breeding Stock Quality Supervision and Testing Center of Ministry of Agriculture,Beijing 100193,China;2.Gansu Agriculture Technology College,Lanzhou 730020,China;3.Sichuan Academy of Grassland Science,Chengdu 611731,China;4.CAAS-ILRI Joint Laboratory on Livestock and Forage Genetic Resources,Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
| Abstract: | A method to distinguish mutton of sheep and goat from duck meat was developed based on the PCR-RFLP analysis of mitochondrial DNA(mtDNA) Cyt b gene.A pair of universal PCR primers was employed to amplify a fragment of mtDNA Cyt b gene in sheep,goat and duck.The PCR products were further digested by two DNA restriction endonucleases of Bsu36I and SpeI.The results showed that the primers amplified successfully a 472bp long DNA fragment from all three species.The PCR-RFLP patterns resulted from SpeI digestion of both sheep and goat PCR products carried two bands in 213bp and 259bp which were different from that of duck PCR product being digested by Bsu36I into 95bp and 377bp.This study demonstrated that the PCR-RFLP analysis of mtDNA Cyt b gene was a rapid method to accurately distinguish mutton of sheep and goat from duck meat. |
| Keywords: | PCR-RFLP species identification mtDNA Cyt b gene |
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