顶空气相色谱法快速测定食用植物油中 有机溶剂残留量的研究

目的建立了顶空气相色谱法检测食用植物油中有机溶剂残留量的分析方法。方法研究了进样的平衡温度、平衡时间,采用优化后的色谱条件,使溶剂残留中的各组分完全分离,减少基质影响,通过记录分离的各组分峰面积,采用归一化法进行定量。结果在2 mg/kg~200 mg/kg浓度范围内,线性关系良好,线性方程为Y=17.4427X+44.4790,R2=0.9999。采用低中高三个浓度的加标回收率均在99.1%~105.0%,相对标准偏差小于3.0%。结论该方法简便、快速、准确,可适用于食用植物油中有机溶剂残留的测定,便于推广应用。

Study on rapid determination of residual solvents in edible vegetable oils by headspace gas chromatography

Objective To establish a method to determine the residual solvents in edible vegetable oils by headspace gas chromatography. Methods The equilibrium temperature and equilibrium time were discussed. Residual solvents were separated completely by the optimized method and matrix effects were reduced. By recording the peak area of each component, the results were quantified by normalization method. Results The linear relationship was good from 2 mg/kg to 200 mg/kg, and the linear equation was Y=17.4427X+44.4790, R2=0.9999. The additive recovery was 99.1%~105.0% and the coefficient of variation was less than 3.0%. Conclusion This method is easier, faster and suitable for the determination of residual solvents in edible vegetable oil, which is convenient for popularization and application.