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| 固体豆粕为氮源根霉12~#产纤溶酶液体发酵条件的优化 | |
| 引用本文： | 李树文,刘晓兰,杜连祥,咸洪泉.固体豆粕为氮源根霉12~#产纤溶酶液体发酵条件的优化[J].中国酿造,2010(1). |
| 作者姓名： | 李树文 刘晓兰 杜连祥 咸洪泉 |
| 作者单位： | 1. 青岛农业大学,生命科学学院,山东,青岛,266109 2. 齐齐哈尔大学生命科学与工程学院,黑龙江,齐齐哈尔,161006 3. 天津科技大学生物工程学院,天津,300222 |
| 基金项目： | 黑龙江省自然科学基金资助项目 |
| 摘 要： | 以固体豆粕为氮源,通过单因素、Plackett-Burman试验设计和正交试验,从无机氮源、胰蛋白胨、初始pH值、接种量和发酵周期方面优化了中华根霉12#产纤溶酶的液体发酵条件.试验结果表明,该菌株液体发酵产纤溶酶的适宜培养基组成为:麸皮水5%,豆粕粉6%,NaNO30.2%,NH4Cl 0.1%,胰蛋白胨2.5%,初始pH4.3;适宜的培养条件是:接种量20%,发酵周期60h,纤溶酶酶活力为182.00U/mL. |
| 关 键 词： | 中华根霉 纤溶酶 液体发酵 |
Optimization of liquid fermentation conditions for fibrinolysin production by Rhizopus chinesis 12# using soybean cake as nitrogen source |
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| LI Shuwen,LIU Xiaolan,DU Lianxiang,XIAN Hongquan.Optimization of liquid fermentation conditions for fibrinolysin production by Rhizopus chinesis 12# using soybean cake as nitrogen source[J].China Brewing,2010(1). | |
| Authors: | LI Shuwen LIU Xiaolan DU Lianxiang XIAN Hongquan |
| Affiliation: | LI Shuwen1,LIU Xiaolan2,DU Lianxiang3,XIAN Hongquan1 (1.College of Life Science,Qingdao Agricultural University,Qingdao 266109,China,2.College of Life Science , Engineering,Qiqihar University,Qiqihar 161006,3.College of Biotechnology,Tianjin University of Science , Technology,Tianjin 300222,China) |
| Abstract: | Liquid fermentation conditions for fibrinolysin production by Rhizopus chinesis 12# choosing soybean cake powder as organic nitrogen source was optimized by single-factor test,Plackett-Burman design and orthogonal test in terms of inorganic nitrogen source,tryptone,initial pH,inoculum and fermentation period.The results showed that the optimum medium consisted of wheat bran 5%,soybean cake power 6%,NaNO3 0.2%,NH4Cl 0.1% and tryptone 2.5% and adjusted pH value to 4.3.The optimum inoculum and fermentation dur... |
| Keywords: | Rhizopus chinesis fibrinolysin liquid fermentation |
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