| 茶叶中甲胺磷农药快速测定研究 |
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| 引用本文： | 鞠兴荣,吴定,刘长鹏,高瑀珑,袁建,朱月芳,姚明兰. 茶叶中甲胺磷农药快速测定研究[J]. 食品科学, 2006, 27(8): 205-207 |
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| 作者姓名： | 鞠兴荣 吴定 刘长鹏 高瑀珑 袁建 朱月芳 姚明兰 |
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| 作者单位： | 南京财经大学食品科学与工程学院江苏省粮油品质监控与深加工重点实验室国家粮食局粮油品控检测中心 |
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| 基金项目： | 江苏省科技厅农业高新技术产业项目;江苏省教育厅高新技术产业化项目 |
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| 摘 要： | 利用有机磷农药能抑制植物酯酶酶促反应的机理,建立了快速测定茶叶中甲胺磷农药残留的方法。用70%饱和硫酸铵对面粉中植物酯酶进行纯化,在45℃、pH4.5～5.5时制备的酶催化反应活性最大,酶催化反应动力学显示10min内反应呈线性关系。测定甲胺磷农药残留反应体系最大吸收峰是在480nm之间,最适乙酸-β-萘酯用量为1.0ml;在甲胺磷浓度在0.1～1.0mg/kg之间时,反应体系的吸光值与甲胺磷浓度呈线性相关;在茶叶汁提取液回收率为84%。
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| 关 键 词： | 茶叶 甲胺磷 快速测定 |
| 文章编号： | 1002-6630（2006）08-0205-03 |
| 收稿时间： | 2006-04-30 |
| 修稿时间： | 2006-04-30 |
Methamidophos Residue Determination in Tea |
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| JU Xing-rong,WU Ding,LIU Chang-peng,GAO Yu-long,YUAN Jian,ZHU Yue-fang,YAO Ming-lan. Methamidophos Residue Determination in Tea[J]. Food Science, 2006, 27(8): 205-207 |
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| Authors: | JU Xing-rong WU Ding LIU Chang-peng GAO Yu-long YUAN Jian ZHU Yue-fang YAO Ming-lan |
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| Affiliation: | College of Food Science and Engineering,Nanjing University of Finance and Economics,Key Laboratory of Grain and Oil Quality Control and Deep-utilizing Technology of Jiangsu Province,Detecting Technology and Engineering Research Center of National Grain Department for Grain and Oil Quality,Nanjing 210003,China |
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| Abstract: | The rapid method of methamidophos residues was developed on the principle that the organophosphorus pesticide can inhibit the plant-esterase activity.The plant-esterase from wheat flour were pureed by 70% saturated ammonium sulfate,with the maximum activity at 45℃ and pH4.5～5.5,and the line relation both reactive velocity and reactive time in 10min.The reactive system of determination shows the maximum peak on 480nm,with optimal content of 1.0ml for acetic-β-naphathone,the line relation both absorption value and methamidophos concentration in range of 0.10～1.0mg/kg,the recovery ratio of 84% for tea juice. |
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| Keywords: | tea methamidophos rapid determination |
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