Potential Development of Vitrified Immature Human Oocytes: Influence of the Culture Medium and the Timing of Vitrification |
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| Authors: | Irene Peinado,Isabel Moya,Laura Garcí a-Valverde,Raquel Francé s,Rosana Ribes,Patrocinio Polo,Marí a José Gó mez-Torres,Ana Monzó |
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| Affiliation: | 1.Assisted Human Reproduction, La Fe University and Polytechnic Hospital, 46026 Valencia, Spain;2.Biotechnology Department, Alicante University, 03690 Alicante, Spain;3.Energy and Memory, Brain Plasticity Unit, CNRS, ESPCI Paris, PSL Research University, 75005 Paris, France;4.Cátedra Human Fertility, Alicante University, 03690 Alicante, Spain |
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| Abstract: | How does the in vitro maturation (IVM) medium and the vitrification procedure affect the survival of germinal vesicle (GV) oocytes obtained from stimulated cycles and their development to the blastocyst stage? In total, 1085 GV human oocytes were obtained after women underwent a cycle of controlled ovarian stimulation, and these oocytes were subjected to IVM before or after their vitrification. IVM was carried out in two commercial culture media not specifically designed for maturation. MII oocytes were then activated and embryo development until day 6 was evaluated. According to the results, a higher percentage of oocytes reach the MII stage if they are vitrified before they undergo IVM. Nevertheless, the medium used and the sample size determine whether these differences become significant or not. Similar survival rates and development to blastocysts were observed in all the conditions studied. |
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| Keywords: | germinal vesicle oocyte in vitro maturation cryopreservation embryo development vitrification survival |
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