| 模拟移动床连续分离L-苯丙氨酸的研究 |
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| 引用本文： | 吴昊,韦萍,万红贵,何若平,于荣华,张赣道.模拟移动床连续分离L-苯丙氨酸的研究[J].食品科学,2007,28(4):64-68. |
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| 作者姓名： | 吴昊 韦萍 万红贵 何若平 于荣华 张赣道 |
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| 作者单位： | 南京工业大学制药与生命科学学院； 南京工业大学制药与生命科学学院 江苏 南京 210009； 江苏 南京 210009； |
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| 基金项目： | 国家自然科学基金;国家重点基础研究发展计划(973计划) |
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| 摘 要： | 利用模拟移动床结合离子交换法从酶转化液中连续分离L-苯丙氨酸。在模拟移动床分离过程中,L-苯丙氨酸的吸附波稳定在区Ⅲ,吸附损失为零,单位质量树脂对L-苯丙氨酸的吸附量可达到125g/kg树脂,提高了树脂利用率:利用区Ⅰ的解吸尾流解吸杂质,L-天冬氨酸、蛋白等杂质的解吸波稳定在区Ⅱ,先于L-苯丙氨酸脱离模拟移动床分离系统;L-苯丙氨酸在区Ⅰ被集中解吸,解吸液中L-苯丙氨酸的浓度达35g/L。实验结果表明该法的L-苯丙氨酸解吸收率达97．6%,单位质量L-Phe的氨水(0．5mol/L)消耗率仅为38．37L/kg。
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| 关 键 词： | L-苯丙氨酸 模拟移动床 离子交换 吸附 解吸 |
| 文章编号： | 1002-6630（2007）04-0064-05 |
| 修稿时间： | 2006-05-17 |
Study on L-phenylalanine Separation by Simulated Moving Bed |
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| WU Hao,WEI Ping,WAN Hong-gui,HE Ruo-ping,YU Rong-hua,ZHANG Gan-dao.Study on L-phenylalanine Separation by Simulated Moving Bed[J].Food Science,2007,28(4):64-68. |
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| Authors: | WU Hao WEI Ping WAN Hong-gui HE Ruo-ping YU Rong-hua ZHANG Gan-dao |
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| Affiliation: | College of Life Science and Pharmacy,Nanjing University of Technology,Nanjing 210009,China |
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| Abstract: | A new separation method of L-phenylalanine(L-Phe)from liquid of transformation was studied,and based on ion exchange and continuous separation by simulated moving bed(SMB)with standing wave design.The results showed that adsorption wave of L-Phe stays in zoneⅢwithout any adsorption loss,and the adsorptive capacity of L-Phe is 125g/kg resin, wheres the utilization of resin is improved.Desorption waves of asparagic acid and protein stay in zoneⅡby wake flow of the extract firm zoneⅠand desorption wave of L-Phe stays in zone L The L-Phe concentration of elution is above 35g/L,while 97.6% L-Phe is obtained from desorption by simulated moving bed with ammonia consumption(0.5mol/L)rate of L-Phe only 38.37L/kg. |
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| Keywords: | L-phenylalanine simulated moving bed ion exchange adsorption desorption |
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