| 脂肪酶产生菌的筛选及其耐甲醇驯化的研究 |
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| 引用本文： | 王珊,孙凤麟,孙玉梅,陈莉. 脂肪酶产生菌的筛选及其耐甲醇驯化的研究[J]. 大连轻工业学院学报, 2010, 0(2) |
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| 作者姓名： | 王珊 孙凤麟 孙玉梅 陈莉 |
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| 作者单位： | 大连工业大学生物与食品工程学院; |
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| 基金项目： | 辽宁省教育厅创新团队项目(2006T030) |
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| 摘 要： | 从土样中分离出34株脂肪酶产生菌,在维多利亚蓝培养基上初筛出透明圈与菌落直径比值较大的6株菌,复筛出产脂肪酶活性较高的菌株FM-1,其所产脂肪酶主要为胞内酶,产脂肪酶活力为132 U/g cell。通过反复的驯化培养方式将菌株FM-1驯化成耐甲醇的优良菌株。在甲醇质量浓度为11 mg/L的培养基中驯化后的脂肪酶相对活力为35%,而出发菌株在此环境中不能生长。
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| 关 键 词： | 脂肪酶产生菌 筛选 甲醇耐受性 驯化 |
Screen and acclimation tolerant to methanol of strains producing lipase |
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| WANG Shan,SUN Feng-lin,SUN Yu-mei,CHEN Li. Screen and acclimation tolerant to methanol of strains producing lipase[J]. Journal of Dalian Institute of Light Industry, 2010, 0(2) |
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| Authors: | WANG Shan SUN Feng-lin SUN Yu-mei CHEN Li |
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| Affiliation: | WANG Shan,SUN Feng-lin,SUN Yu-mei,CHEN Li(School of Biological & Food Engineering,Dalian Polytechnic University,Dalian 116034,China) |
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| Abstract: | The lipase-producing strains were isolated from soils.Six strains out of the 34 isolates were selected by the larger transparent zone on the selective medium with victoria blue.Strain FM-1 was the best strain producing intracellular lipase with high activity 132 U/g cell.The strain FM-1 was acclimated repeatedly by the traditional cultivation in the medium containing mehanol,which became the strain tolerant to methanol.After acclimation in the medium containing 11 mg/L methanol,the relative lipase activity ... |
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| Keywords: | strains producing lipase screen tolerant to methanol acclimation |
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