| 人VEGF受体II基因3区的克隆、表达研究 |
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| 引用本文： | 熊冬生,刘嘉,邵晓枫,李蓉,许元富,彭晖,范冬梅,朱祯平,杨纯正. 人VEGF受体II基因3区的克隆、表达研究[J]. 高技术通讯, 2002, 12(5): 45-49 |
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| 作者姓名： | 熊冬生 刘嘉 邵晓枫 李蓉 许元富 彭晖 范冬梅 朱祯平 杨纯正 |
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| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所实验血液学,国家重点实验室,天津,300020 |
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| 基金项目： | 国家攀登计划 (95 专 10 )，天津市重大科技攻关 (0 0 3 1195 11)资助项目 |
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| 摘 要： | 用RT PCR法从人胎儿脐静脉内皮细胞中克隆人VEGF受体II基因 3区 ,将其重组到 pAYZ表达载体中 ,构建成人VEGF受体II基因 3区表达载体。将该载体转化大肠杆菌 16C9,获得稳定表达 ,表达产物以可溶性状态存在 ;SDS PAGE和Westernblot分析显示 ,其分子量约为 14kD。以纯化的表达产物免疫Balb/c小鼠 ,眼球采血制备抗血清 ,抗血清效价在 10 3以上 ,并具有抑制VEGF诱导内皮细胞增殖的作用 ,在抑制肿瘤血管新生中具有潜在的应用前景。
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| 关 键 词： | 血管内皮细胞生长因子 血管内皮细胞生长因子受体 克隆 表达 |
| 修稿时间： | 2002-02-06 |
Cloning and Expression of Domain3 of VEGF Receptor II |
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| Xiong Dongsheng,Liu Jia,Shao Xiaofeng. Cloning and Expression of Domain3 of VEGF Receptor II[J]. High Technology Letters, 2002, 12(5): 45-49 |
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| Authors: | Xiong Dongsheng Liu Jia Shao Xiaofeng |
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| Abstract: | RT PCR was used to clone DNA fragment of domain3 of VEGF receptor II (KDRIII ) from human umbilical vein endothelial cell, and the expression vector of KDRIII was constructed by cloning KDRIII DNA fragment into the expression vector pAYZ KDRIII was expressed in E coli 16C9 and purified by affinity chromatography The serum against KDRIII was prepared from mice immunized with a soluble form of KDRIII The serum against KDRIII suppressed proliferation of ECV307 induced by VEGF and have potential clinical application in the treatment of cancer where pathological angiogenesis is involved |
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| Keywords: | VEGF KDR Cloning Expression |
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