| 基体辅助激光解吸电离飞行时间质谱仪的离子延时引出新技术研究 |
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| 引用本文： | 赵善楷,何维得,查庆民. 基体辅助激光解吸电离飞行时间质谱仪的离子延时引出新技术研究[J]. 现代仪器, 2001, 0(2): 1-3 |
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| 作者姓名： | 赵善楷 何维得 查庆民 |
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| 作者单位： | 中山大学测试中心,广州,510275 |
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| 基金项目： | 中国分析测试协会科学基金资助项目 |
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| 摘 要： | 为了提高基体辅助激先解吸电离飞行时间质谱仪(MALDI-TOF-MS)的分辨率,在自行研制的研究级激光飞行时间质谱仪上进行了离子延时引出(Delay Extraction,DE)新技术的实验研究,建立了新结构离子源和离子延迟引出的电子控制系统及相应软件。通过电场模拟和实验,研究了样品相对质量、延迟引出时间、引出电压对提高分辨率的影响。在使用40MH_z瞬态记录仪的情况下,测得环十肽(m/z=1214)分辨率为1734,胰岛素(m/z=5375)分辨率为2124。
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| 关 键 词： | 激光飞行时间质谱仪 延时引出 高分辨率 |
A Study on Delayed Ion Extraction of Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry |
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| Zhao Shankai He Weide Zha Qingmin. A Study on Delayed Ion Extraction of Matrix-assisted Laser Desorption Ionization Time-of-flight Mass Spectrometry[J]. Modern Instruments, 2001, 0(2): 1-3 |
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| Authors: | Zhao Shankai He Weide Zha Qingmin |
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| Abstract: | In this paper a study on delayed ion extraction in laser desorption/ionization time-of-flight mass spectrometry is described, which gives a much higher mass resolution compared with that the of previous continuous mode.By computer modeling and carefully selecting the parameters such as delayed time and voltage ratio (extracting volt/repelling volt) ,we have obtained the mass resolution up to 1734(reflect mode) with gramicidine (α-cyano-4-hydroxycinnamic acid as matrix) ;2124 with insulin(4HCCA as matrix) on our modified laboratory-built instrument. |
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| Keywords: | laser desorption/ionizadon time-of-flight mass spectrometry delayed ion extraction |
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