Wax ester-synthesizing activity of lipases |
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Authors: | Takahiro Tsujita Maho Sumiyoshi Hiromichi Okuda |
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Affiliation: | (1) Department of Medical Biochemistry, School of Medicine, Ehime University, 791-0295 Ehime, Japan;(2) Central Research Laboratory, School of Medicine, Ehime University, Shigenobu, Onsen-gun, 791-0295 Ehime, Japan |
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Abstract: | The synthesis/hydrolysis of wax esters was studied in an aqueous solution using purified rat pancreatic lipase, porcine pancreatic
carboxylester lipase, and Pseudomonas fluorescens lipase. The equilibrium between wax ester synthesis and hydrolysis favored ester formation at neutral pH. The synthesizing
activities were measured using free fatty acid or triacylglycerol as the acyl donor and an equimolar amount of long-chain
alcohol as the acyl acceptor. When oleic acid and hexadecanol emulsified with gum arabic were incubated with these lipases,
was ester was synthesized, in a dose- and time-dependent manner, and the apparent equilibrium ratio of palmityl oleate/free
oleic acid was about 0.9/0.1. These lipases catalyzed the hydrolysis of palmityl oleate emulsified with gum arabic, and the
apparent equilibrium ratio of palmityl oleate/free oleic acid was also about 0.9/0.1. The apparent equilibrium ratio of wax
ester/free fatty acid catalyzed by lipase depended on incubation pH and fatty alcohol chain length. When equimolar amounts
of trioleoylglycerol and fatty acyl alcohol were incubated with pancreatic lipase, carboxylester lipase, or P. fluorescens lipase, wax esters were synthesized dose-dependently. These results suggest that lipases can catalyze the synthesis of wax
esters from free fatty acids or through degradation of triacylglycerol in an aqueous medium. |
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