Real-time PCR-based detection and quantification of genetically modified maize in processed feeds commercialised in Malaysia |
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| Affiliation: | 1. Department of Chemistry, Ministry of Science, Technology and Innovation Malaysia, Jalan Sultan, 46661 Petaling Jaya, Selangor, Malaysia;2. Centre of Excellence for Food Safety Research, Faculty of Food Science and Technology, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia;3. Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, 43400 Serdang, Selangor, Malaysia;1. School of Veterinary & Life Sciences, Molecular and Biomedical Sciences, Vector- and Water-Borne Pathogen Research Group, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia;2. ANU Medical School, The Australian National University, Linnaeus Way, Canberra, ACT 0200, Australia;3. Canberra Hospital Campus, The Canberra Hospital, Hospital Road, Garran, ACT 2606, Australia;1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China;2. State Key Laboratory of Animal Nutrition, Beijing 100193, P.R.China;1. Scientific Institute of Public Health (WIV-ISP), Platform of Biotechnology and Molecular Biology (PBB) and Biosafety and Biotechnology Unit (SBB), J. Wytsmanstraat 14, 1050 Brussels, Belgium;2. Institute for Agricultural and Fisheries Research (ILVO), Technology and Food Sciences Unit, Burg. Van Gansberghelaan 115 bus 1, 9820 Merelbeke, Belgium;3. Walloon Agricultural Research Centre (CRA-W), Unit Traceability and Authentication, Chaussée de Namur 24, 5030 Gembloux, Belgium |
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| Abstract: | The present study which dealt mainly with processed feeds and some maize samples sold commercially in Malaysia evaluated the implementation of a real-time PCR cycling system for singleplex screening of eight target sequences (lectin, hmg, adh1, p35S, NK603, GA21, MON810 and MON863) and quantification of four genetically modified (GM) maize events (NK603, GA21, MON810 and MON863). The effects of using proprietary glass magnetic particles to bind DNA to their surface were also investigated in terms of DNA quantity, purity, integrity, quality and its overall effect on DNA amplification. GM material was present in 26.2% feeds and 65% maize samples. All GM samples contained MON810 followed by NK603 (47.5%), GA21 (25%) and MON863 (2.5%). Single-event and multiple-events were identified in the GM samples with 50% of the GM samples containing multiple-events. The present study which represents a fast and reliable methodology would provide an overview of the presence and levels of GMOs in feeds and maize in Malaysia. |
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