Microfluidic device with dual-channel fluorescence acquisition for quantification/identification of cancer cells |
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| Authors: | Eric Pedrol Javier Martínez Magdalena Aguiló Manuel Garcia-Algar Moritz Nazarenus Luca Guerrini Eduardo Garcia-Rico Francesc Díaz Jaume Massons |
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| Affiliation: | 1.Física i Cristal·lografia de Materials i Nanomaterials and EmaS,Universitat Rovira i Virgili,Tarragona,Spain;2.Departamento de Química Física e Inorgánica and EmaS,Universitat Rovira i Virgili,Tarragona,Spain;3.Fundacion de Investigacion HM Hospitales,Madrid,Spain;4.Centro Integral Oncologico Clara Campal (CIOCC),Madrid,Spain;5.Department of Medical Oncology,Hospital Universitario HM Torrelodones,Torrelodones,Spain;6.School of Medicine,San Pablo CEU,Madrid,Spain;7.ICREA,Barcelona,Spain |
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| Abstract: | This paper presents an optofluidic device for cell discrimination with two independent interrogation regions. Pumping light is coupled to the device, and cell fluorescence is extracted from the two interrogation zones by using optical fibers embedded in the optofluidic chip. To test the reliability of this device, AU-565 cells—expressing EpCAM and HER2 receptors—and RAMOS cells were mixed in a controlled manner, confined inside a hydrodynamic focused flow in the microfluidic chip and detected individually so that they could be discriminated as positive (signal reception from fluorescently labeled antibodies from the AU-565 cells) or negative events (RAMOS cells). A correlation analysis of the two signals reduces the influence of noise on the overall data. |
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