鸡蛋蛋白质含量无损检测技术研究

为了探索傅立叶近红外光谱（FT-NIR）快速无损检测鸡蛋蛋白质的方法,采集83枚新鲜种鸡蛋赤道位和锐端的近红外光谱（12000⁴000cm-1）,用凯氏定氮法测定对应鸡蛋的粗蛋白含量,运用The Unscrambler软件中的一阶导数（First Derivative,FD）、二阶导数（Second Derivative,SD）、多元散射校正（Multiplication Scatter Correction,MSC）、变量标准化（Standard Normalized Variate,SNV）和光谱转换（Spectroscopic Transformation,ST）方法预处理光谱,结合偏最小二乘法（PLS）建立蛋白质的数学模型并用杠杆校正（Leverage Correction）检验,通过模型的比较来判断FT-NIR快速无损检测鸡蛋蛋白质的可行性。结果表明:鸡蛋蛋白质与其近红外光谱信号间存在线性关系,在未作预处理下用赤道位和锐端原始光谱建立的蛋白质校正模型R2在0.74以上;在不同预处理中,SD处理下建立模型效果最优,赤道位和锐端模型的R2均在0.97以上,RMSEE、RMSEP值都较小且相互接近;SD处理下建立的赤道位和锐端光谱模型差异不显著,R2及RMSEE、RMSEP都较为接近。利用FT-NIR快速无损检测鸡蛋蛋白质的含量是可行的。 

Study on the egg protein content with nondestructive detection technology

In order to explore the Fourier transform near- infrared spectroscopy ( FT- NIR) rapid non- destructive detection methods of egg protein, the near- infrared spectroscopy ( 12000⁴000cm- 1) of 83 fresh breeding eggs in equatorial side and sharp side were collected, then the Kjeldahl method was used to determine the crude protein content of correspond eggs, and by First Derivative ( FD) , Second Derivative ( SD) , Multiplication Scatter Correction ( MSC) , Standard Normalized Variate ( SNV) and Spectroscopic Transformation ( ST) of The Unscrambler pretreated spectroscopy. Combined with partial least squares ( PLS) method with leverage correction to establish the mathematical model of the protein, and through the model to determine the feasibility of FT- NIR rapid non-destructive inspection of egg protein. The results showed that: Linear relationship exists between the egg protein and its near- infrared spectroscopy signal, the protein correction model with the equatorial and sharp side original spectrum above 0.74; In the different pretreatment, the model with SD treatment was best effect, equatorial position and sharp- side model R2 were above 0.97, values of RMSEE, RMSEP were small and close to each other; With the treatment of SD, equatorial position and the sharp- side spectrum model had no significant difference, R2, RMSEE, RMSEP were closer.By using FT- NIR rapid non- destructive detection the content of egg protein was feasible.