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毛蚶抗菌活性筛选及低极性组分GCMS分析
引用本文:徐艳, 张秀国, 黄国强, 张琴, 杨家林, 孙雪萍. 毛蚶抗菌活性筛选及低极性组分GCMS分析[J]. 食品工业科技, 2015, (11): 127-130. DOI: 10.13386/j.issn1002-0306.2015.11.017
作者姓名:徐艳  张秀国  黄国强  张琴  杨家林  孙雪萍
作者单位:1. 西海洋研究所,海洋生物技术重点实验室,广西北海536000
2. 北京航空航天大学北海学院,广西北海,536000
摘    要:目的:研究毛蚶的抗菌活性部位及有效成分。方法:毛蚶软组织经乙醇提取后,采用液液萃取法将提取物分为石油醚相、乙酸乙酯相、正丁醇相和水相4个不同的极性部位;采用抑菌圈法和最小杀菌浓度测定法,对毛蚶乙醇提取物和各极性部位进行了抗菌活性测定,并用正相硅胶柱对抗菌活性较好的石油醚相进行了分离纯化,得到甾体组分;采用GC-MS技术对石油醚相和甾体组分进行了化学成分分析。结果:毛蚶石油醚相的抑菌效果最好;从毛蚶石油醚相中鉴定出了30个化合物,主要为脂肪类和甾醇类化合物;从甾体组分中鉴定出8个化合物,其中7个为首次从毛蚶中分离鉴定。结论:毛蚶的石油醚相具有明显的抗菌活性,石油醚相以甾醇类化合物为主。 

关 键 词:毛蚶  抗菌活性  气相色谱-质谱法  甾醇
收稿时间:2014-08-06

Antibacterial activities and GC-MS analysis of low polar components from Scapharca subcrenata Lischke
XU Yan, ZHANG Xiu-guo, HUANG Guo-qiang, ZHANG Qin, YANG Jia-lin, SUN Xue-ping. Antibacterial activities and GC- MS analysis of low polar components from Scapharca subcrenata Lischke[J]. Science and Technology of Food Industry, 2015, (11): 127-130. DOI: 10.13386/j.issn1002-0306.2015.11.017
Authors:XU Yan  ZHANG Xiu-guo  HUANG Guo-qiang  ZHANG Qin  YANG Jia-lin  SUN Xue-ping
Affiliation:1.Guangxi Institute of Oceanology, Guangxi Key Laboratory of Marine Biotechnology;2.Beihai College of Beihang University
Abstract:Objective: To screen the antibacterial fractions and constituents of Scapharca subcrenata Lischke.Methods: The soft tissues of S.subcrenata was extracted by ethanol.Liquid- liquid extraction with petroleum ether, ethyl acetate, n- butanol and water was used for the separation of ethanolic extract and 4 organic fractions were obtained.The antibacterial activities of the ethanolic extract and organic fractions was evaluated by diameter of inhibition zone ( DIZ) and the minimum bactericidal concentration ( MBC) . The active petroleum ether fraction was separated and purified with silica gel column chromatography, and a sterol mixture was obtained.The compounds in the petroleum ether fraction and the sterols were analyzed by gas chromatography- mass spectrometry ( GCMS) .Results: The antibacterial substances were mainly in the petroleum ether fraction, and 30 compounds were identified by GC- MS, most of which were 1ipids and sterols.Eight compounds were identified by GC- MS from the sterol component, and seven compounds were identified for the first time from S. subcrenata. Conclusion: The antibacterial activities of petroleum ether fraction from S. subcrenata were obvious, and sterol was the major compound of petroleum ether fraction.
Keywords:Scapharca subcrenata Lischke  antibacterial activity  gas chromatography-mass spectrometry  sterols
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