响应面法优化超声波辅助提取百合花秋水仙碱工艺

以百合花为研究对象,利用响应面分析法优化百合花中秋水仙碱的超声辅助提取工艺。通过单因素实验分别考察乙醇体积分数、提取时间、提取温度和料液比对百合花秋水仙碱含量的影响。选取适当的实验因素水平,利用Design Expert软件和Box-Behnken设计法设计响应面实验,对各个因素的显著性和交互作用进行分析。结果表明超声提取百合花秋水仙碱的最佳工艺条件为:60%乙醇为提取溶剂、温度50℃、料液比为1∶21、提取时间为33min,该条件下提取的秋水仙碱含量为16.25μg/g。 

Optimization of ultrasonic-assisted extraction process of colchicine from lily flower by response surface methodology

Lily flower as the research object, using the response surface analysis methodology the ultrasonic extraction technology of colchicine was optimazed. On the basis of single-factor tests, the effects of ethanol concentration, extraction duration, extraction temperature, and liquid-material ratio on colchicine content were explored. A regression model describing extraction of colchicine as a function of three variables was established with the Design-Expert software according to the Box-Behnken design principle and experimental data. The results showed that the optimal technologies were 60% ethanol as extraction solvent, extraction temperature50℃, material liquid ratio 1∶21 and extraction time 33 min. Under the optimum condition lily alkaloid extracting yield was 16.25μg/g.