罗非鱼鱼肉及组分蛋白酶解产物的抗氧化特性

采用胃蛋白酶、中性蛋白酶、Alcalase2.4L碱性蛋白酶分别酶解罗非鱼肉蛋白,研究比较不同蛋白酶在1、2、4、6、8h对罗非鱼肉蛋白的酶解效果及产物的抗氧化活性,结果表明:3种酶的酶解产物都具有一定的抗氧化活性,中性蛋白酶酶解产物的水解度和蛋白回收率最高,胃蛋白酶酶解产物对DPPH自由基和羟自由基的清除效果最好;综合各项指标选择中性蛋白酶对罗非鱼肉的组分蛋白(肌原纤维蛋白、肌浆蛋白、基质蛋白)进行4h酶解,研究比较不同组分蛋白的酶解效果及产物的抗氧化活性,结果表明:组分蛋白中基质蛋白酶解产物的蛋白回收率最高,为89.8%,肌原纤维蛋白酶解产物的水解度最高,为10.1%,肌浆蛋白酶解产物的抗氧化活性最好,羟自由基和DPPH自由基的IC50值分别为12.352mg/m L和3.554mg/m L。 

Antioxidant activities of tilapia(Oreochromis niloticus) protein and component proteins hydrolysates

Pepsin, neutral protease, Alcalase2.4L were used to hydrolyse the tilapia protein, respectively. The effect of hydrolysis and antioxidant activities including 1, 1-diphenyl-2-picrylhydrazine (DPPH·) and hydroxyl radical (·OH) of hydrolysates hydrolysed by different proteases for 1, 2, 4, 6, 8h were studied and compared, the results showed that the hydrolysates of three proteases all had antioxidant activities, the hydrolysate of neutral protease had the highest degree of hydrolysis (DH) and the recovery rate of protein, the hydrolysate of pepsin had the minimum IC50 value of DPPH· and ·OH scavenging ability. Neutral protease was selected to hydrolyse sarcoplasmic protein, myofibrillar protein and stroma protein for 4h, the effect of hydrolysis and antioxidant activities of hydrolysates were studied and compared. The results indicated that the hydrolysate of stroma protein had the highest recovery rate of protein that was 89.8%, the hydrolysate of myofibrillar protein had the highest DH that was 10.1%, the hydrolysate of sarcoplasmic protein had the minimum IC50 value of ·OH and DPPH· scavenging ability that were 12.352mg/m L and 3.554mg/m L.