新疆野生苹果(Malus sieversii)的总多酚、总黄酮提取物的抗氧化活性研究

以采集于新疆不同地区的20个野生苹果品种为研究对象,福林酚法测总多酚,分光光度计法测定总黄酮,DPPH、ABTS、还原能力法测定总抗氧化能力,相关系数法分析各个指标之间的相关性。结果显示,不同品种的野生苹果的总多酚、总黄酮及抗氧化活性之间具有显著差异,其中,HDM-23的总多酚含量最高（4714.33μmol没食子酸/100g鲜果）,XY-31的总黄酮含量最高（2452.84mg芦丁/100g鲜果）。总多酚、总黄酮及抗氧化活性之间呈极显著（p<0.01）正相关,表明总多酚与总黄酮是野生苹果抗氧化活性的主要贡献物质。 

Antioxidant activity of polyphenolics and flavonoid extracted from wild apple (Malus sieversii)

Twenty different lines of wild apple (Malus sieversii) collected from different places in Xinjiang, China were used as research objects. Total total polyphenol content was measured using the Folin- Ciocalteau method. Total flavonoid content was measured by spectrophotometer. Total antioxidant capacities were analyzed by ABTS assay, DPPH assay, and iron ( Ⅲ) to iron ( Ⅱ) reducing power assay, and the correlation between the various indicators was measured with Pearson correlation coefficient. It was found that HDM-23 had the highest total polyphenol content (4714.33μmol gallic acid/100 g fresh matter) , and XY-31 contained the highest amount of total flavonoid content (2452.84 mg rutin/100 g fresh matter) . The present study implied that total polyphenols contribute to the antioxidant properties of the wild apple (p<0.01) and total flavonoid was an important phenolic compound in the wild apple varieties investigated.