吲哚乙酸对冬虫夏草生物量、胞外多糖产量及其抗氧化性能的影响

通过在液体培养基中添加不同浓度的吲哚乙酸,研究吲哚乙酸对冬虫夏草生物量、胞外多糖产量及其抗氧化性能的影响。结果表明:适宜浓度的吲哚乙酸可以提高冬虫夏草的生物量和胞外多糖产量,并且随吲哚乙酸浓度的增加二者均呈先增后降的变化趋势,当浓度为2.5μg/m L时,生物量和胞外多糖产量均达到最高,分别为18.3g/L和2.9g/L,较不添加吲哚乙酸分别增加了6.3%和15.4%;同时,适宜浓度的吲哚乙酸还可以提高冬虫夏草胞外多糖的抗氧化性能,在1.0μg/m L和2.5μg/m L时,胞外多糖对羟基自由基和DPPH的清除率分别达到最高,为58.9%和56.6%。 

Effects of indole acetic acids(IAA) on biomass,extracellular polysaccharide production and antioxidant activity of Cordyceps sinensis(Berk.) Sacc.

The effect of indole acetic acids (IAA) on biomass, extracellular polysaccharide (EPS) production and antioxidant activity of Cordyceps sinensis were studied in the present work. The results showed that the reasonable concentration of IAA could increase biomass and EPS production of Cordyceps sinensis. Biomass and EPS production were increased first and then decreased with the increase of IAA. Both of biomass and EPS production reached the highest content at 2.5μg/m L IAA, namely 18.3g/L and 2.9g/L, increased 6.3% and15.4% comparing no IAA, respectively. The appropriate concentration of IAA could also improve the antioxidant activity of EPS. The strongest scavenging effect on hydroxyl radical and DPPH of EPS were 58.9% and 56.6%at the concentrations of 1.0μg/m L and 2.5μg/m L, respectively.