枫香叶正丁醇提取物抑菌效果及其抗氧化活性研究

采用打孔法测定抑菌圈大小和试管连续稀释法测定最小抑菌浓度(MIC),研究了枫香叶正丁醇提取物抑菌效果,并测定其对DPPH自由基、超氧阴离子自由基和羟自由基的清除活性。结果表明:枫香叶正丁醇提取物对7种实验菌均有抑制作用,不同浓度间、不同菌种间存在极显著(p<0.01)差异;且对细菌的抑制作用强于真菌,对革兰氏阴性菌的抑菌效果比革兰氏阳性菌好;对大肠杆菌、枯草杆菌、金黄色葡萄球菌、四联球菌、黄曲霉、黑曲霉、青霉的MIC分别为3.1、12.5、12.5、6.25、12.5、25、12.5mg/m L。枫香叶正丁醇提取物具有较强的的抗氧化活性,可作为功能性食品资源开发利用。 

In vitro antioxidant and antimicrobial activity of N-butanol extract from Liquidambar formosana Hance leaves

To explore antioxidant activity and inhibitory effect of N-butanol extract from Liquidambar formosana Hance leaves, in this test determine the size of the inhibition zone using drilling method and the minimum inhibitory concentrations ( MIC) with tubes serial dilution method, then detect and evaluate its antioxidation activities by scavenging activity to DPPH·, superoxide anion (O2-·) and hydroxyl (·OH) free radicals. Results showed that N-butanol extract from Liquidambar formosana Hance leaves had inhibition to seven kinds of tested bacterias and significant differences among different concentrations and different strains. In addition, the inhibition of bacterial was stronger than fungal as well as the antibacterial effect on Gram-negative bacteria was better than Gram-positive bacteria. The MIC of Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Micrococcus tetragenus, Aspergillus flavus, Aspergillus niger, Penicillium oxalicum were 3.1, 12.5, 12.5, 6.25, 12.5, 25, 12.5mg/m L, respectively. The results also showed that the extract from Liquidambar formosana Hance leaves, which had strong antioxidant activities, could be developed as a functional food resource.