| 曲酸对菠萝蜜多酚氧化酶的抑制作用和抑菌实验 |
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| 引用本文： | 何世微, 陶毅明, 王贵平, 廖朝亮. 曲酸对菠萝蜜多酚氧化酶的抑制作用和抑菌实验[J]. 食品工业科技, 2015, (11): 159-161. DOI: 10.13386/j.issn1002-0306.2015.11.023 |
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| 作者姓名： | 何世微 陶毅明 王贵平 廖朝亮 |
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| 作者单位： | 1.桂林医学院生物技术学院 |
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| 摘 要： | 采用酶动力学和Lineweaver-Burk双倒数法作图研究了曲酸对菠萝蜜多酚氧化酶PPO的抑制作用和机理,并通过平板菌落实验研究了曲酸对果肉的抑菌效果。结果表明,曲酸对PPO酶活有很强的抑制作用,其IC50为0.297mmol/L,表现为可逆非竞争性抑制,解离常数Ki为0.296mmol/L;果肉经0.10%曲酸浸泡后6d内具有较好的抑菌效果。
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| 关 键 词： | 曲酸 菠萝蜜 多酚氧化酶 抑制作用 抑菌 |
| 收稿时间： | 2014-07-23 |
Inhibitory effect of kojic acid on polyphenol oxidase from jackfruit and its antibacterial experiment |
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| HE Shi-wei, TAO Yi-ming, WANG Gui-ping, LIAO Chao-liang. Inhibitory effect of kojic acid on polyphenol oxidase from jackfruit and its antibacterial experiment[J]. Science and Technology of Food Industry, 2015, (11): 159-161. DOI: 10.13386/j.issn1002-0306.2015.11.023 |
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| Authors: | HE Shi-wei TAO Yi-ming WANG Gui-ping LIAO Chao-liang |
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| Affiliation: | 1.College of Biotechnology, Guilin Medical University |
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| Abstract: | The inhibitory effect and mechanism of kojic acid on polyphenol oxidase ( PPO) from jackfruit were studied by enzyme kinetics and Lineweaver- Burk plots.The antibacterial effect of kojic acid on jackfruit bulbs was tested by plate counting. The results showed that kojic acid significantly inhibited the enzyme activity, with an IC50of0.297 mmol / L.Kojic acid was a reversible non- competitive inhibitor, with the Kiof 0.296 mmol / L. Jackfruit bulbs dipped in 0.10% kojic acid showed good antibacterial effect within 6d. |
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| Keywords: | kojic acid jackfruit polyphenol oxidase inhibitory effects antibacterial |
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