|
|||||
|
|
| 牛奶过敏原检测方法研究进展 | |
| 引用本文： | 贾敏,张亦凡,张银志,孙秀兰.牛奶过敏原检测方法研究进展[J].食品工业科技,2015,36(12):385-389. |
| 作者姓名： | 贾敏 张亦凡 张银志 孙秀兰 |
| 作者单位： | 1. 江南大学食品学院,江苏无锡,214122 2. 陕西省汉中市产品质量监督检验所,陕西汉中,723000 3. 江南大学食品科学与技术国家重点实验室,江苏无锡,214122 4. 江南大学食品学院,江苏无锡214122;江南大学食品科学与技术国家重点实验室,江苏无锡214122 |
| 摘 要： | 牛奶是八大食物致敏原之一,能引起严重的过敏反应。如何实现食物中牛奶过敏原的快速,准确筛检已成为食品安全领域关注的焦点。牛奶主要过敏原为酪蛋白、α-乳白蛋白、β-乳球蛋白,其检测方法有电泳法、色谱法、酶联免疫实验（ELISA）、传感器、基于质谱的蛋白质组学（LC-MS/MS）、聚合酶链式反应技术（PCR）等。本文就其检测方法的原理和应用进行综述,旨在为食品中牛奶过敏原的检测提供技术方向,以便保障牛奶过敏患者的安全。 |
| 关 键 词： | 牛奶过敏原 检测 酶联免疫实验 聚合酶链式反应技术 基于质谱的蛋白质组学 |
| 收稿时间： | 2014-09-23 |
Research progress of milk allergen detection |
|
| JIA Min,ZHANG Yi-fan,ZHANG Yin-zhi,SUN Xiu-lan.Research progress of milk allergen detection[J].Science and Technology of Food Industry,2015,36(12):385-389. | |
| Authors: | JIA Min ZHANG Yi-fan ZHANG Yin-zhi SUN Xiu-lan |
| Affiliation: | 1.School of Food Science of Jiangnan University;2.Hanzhong Products Quality Supervison and Inspection Institute;3.State Key Laboratory of Food Science and Technology |
| Abstract: | Milk is recognized as one of the major food allergens, which could cause severe allergic reactions.The rapid and accurate detection of milk allergen have gained tremendous attention in the field of food safety.The major allergens in milk include casein (CN) , beta-lactoglobulin (α-Lg) and alpha-lactalbumin (β-La) . The currently available detection method include electrophoresis chromatography, enzyme-linked immunosorbent assay (ELISA) , biosensor, proteomics based on mass spectrometry (LC-MS/MS) and polymerase chain reaction (PCR) . In this article, the principles and applications of major detection methods for milk allergens were summarized, which will provide guidance for the milk allergen detection in different food sources and the ensure of food safety for human health. |
| Keywords: | milk allergen detection ELISA PCR LC-MS/MS |
| 本文献已被 CNKI 等数据库收录！ | |
| 点击此处可从《食品工业科技》浏览原始摘要信息 | |
| 点击此处可从《食品工业科技》下载全文 | |