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超声波辅助提取银杏落叶中多糖的工艺初探
引用本文:郭香琴,蔡娜,杨善彬,周石洋,陈意心,柯道瑶.超声波辅助提取银杏落叶中多糖的工艺初探[J].生物质化学工程,2017,51(6):43-47.
作者姓名:郭香琴  蔡娜  杨善彬  周石洋  陈意心  柯道瑶
作者单位:1. 重庆师范大学 化学学院, 重庆 401331;2. 重庆师范大学 活性物质生物技术教育部工程研究中心, 重庆 401331
基金项目:活性物质生物技术教育部工程研究中心开放基金项目(20150408,AS201607);大学生创新创业训练计划项目(201510637085);重庆高校创新团队项目(CXTDX201601018)
摘    要:以银杏落叶为原料,采用超声波辅助水提醇沉的方法提取银杏落叶中的多糖,通过单因素试验和响应曲面法对银杏落叶中多糖提取的工艺进行初探。结果表明:最佳提取工艺条件为5.0 g银杏落叶粗粉,超声波功率600 W,液料比40∶1(mL∶g),提取温度84 ℃,提取时间50 min,多糖提取得率6.61 %。由于本研究只是对银杏落叶中多糖提取工艺的初步研究,因此选择提取的液料比时未深入考虑"少量多次,总液比尽量少"的提取原则。

关 键 词:银杏落叶  多糖  超声波提取  响应曲面法  
收稿时间:2016-10-16

Preliminary Research on Ultrasonic Assisted Extraction Technology of Polysaccharides in Ginkgo biloba Leaves
GUO Xiangqin,CAI Na,YANG Shanbin,ZHOU Shiyang,CHEN Yixin,KE Daoyao.Preliminary Research on Ultrasonic Assisted Extraction Technology of Polysaccharides in Ginkgo biloba Leaves[J].Biomass Chemical Engineering,2017,51(6):43-47.
Authors:GUO Xiangqin  CAI Na  YANG Shanbin  ZHOU Shiyang  CHEN Yixin  KE Daoyao
Affiliation:1. College of Chemistry, Chongqing Normal University, Chongqing 401331, China;2. Engineering Research Center of Biotechnology of Active Substances(Ministry of Education) in Chongqing Normal University, Chongqing 401331, China
Abstract:Polysaccharide was extracted from Ginkgo biloba fallen leaves by ultrasound-assisted water extraction and alcohol precipitation. The extraction technology was preliminarily studied by single factor and response surface method.The results showed that the optimum extraction conditions were as follows:G. biloba fallen leaves 5.0 g, ultrasound power 600 W, liquid/solid ratio 40:1(mL:g), extraction temperature 84 ℃, extraction time 50 min, the final polysaccharide yield was 6.61 %. Because this study was only a preliminary study on the extraction process of polysaccharides from the leaves of Ginkgo biloba, the extraction principle of "small amount of times and total liquid ratio is less" was not considered.
Keywords:Ginkgo biloba fallen leaves  polysaccharide  ultrasonic extraction  response surface method  
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