Analysis of ATP and Its Breakdown Products in Beef by Reversed-Phase HPLC |
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| Authors: | AKIRA WATANABE EISAKU TSUNEISHI YUJI TAKIMOTO |
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| Affiliation: | The authors are with the Tohoku National Agricultural Experiment Station, 4 Akahira, Shimo-kuriyagawa, Morioka, Iwate Prefecture 020-01 Japan. |
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| Abstract: | Analytical conditions for the quantitative determination of adenosine 5′-triphosphate(ATP), adenosine 5′-diphosphate(ADP), inosine 5′-monophosphate(IMP), adenosine 5′-monophosphate(AMP), hypoxanthine(Hyp), xanthine(Xan), inosine(Ino) and adenosine(Ado) in meat extracts by reversed-phase high-performance liquid chromatography (HPLC) were examined. A commercial ODS column with a 5-μm particle diameter was used, and expeimental parameters affecting the separation were discussed. Peaks in chromatograms of meat extracts were identified by retention time, co-injection with standards, absorbance ratios and the enzymatic peak shift method. The procedure proposed was adaptable as an indication of freshness of meat. |
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