玫瑰花总黄酮微波辅助提取及其抗氧化研究

采用微波辅助提取的方法提取玫瑰花中的总黄酮,并对提取的黄酮进行了抗氧化测定。结果表明:玫瑰花总黄酮含量为2.2%,最佳提取工艺条件为提取温度65℃、料液比1∶55、乙醇浓度65%、微波时间45min、微波功率700W,提取率为82.29%。清除DPPH·实验测得玫瑰花黄酮的乙酸乙酯、正丁醇、水萃取物的IC50分别为6.52、9.04、6.87μg/mL;清除O2-.实验测得在萃取物浓度为100μg/mL时,乙酸乙酯、正丁醇、水萃取物对O2-·的抑制率分别为14.43%、27.83%、23.21%;清除.OH的实验测得在萃取物浓度为500μg/mL时,乙酸乙酯、正丁醇、水萃取物对.OH的抑制率分别为23.74%、43.51%、32.75%。实验得到结论为玫瑰花中的黄酮类化合物具有一定的抗氧化活性。

Microwave- assisted extraction and antioxidant activity of flavonoids from rose

The microwave-assisted extraction of flavonoids from rose and its oxidation resistance were investigated. The results showed that the optimal temperature, ratio of liquid to solid, alcohol concentration, microwave extraction time and microwave power were 65℃, 1 : 55,65%, 45min and 700W, respectively, and the extraction ratio for flavonoids was 82.29% .The obtained total flavonoids content was 2.2%. The antioxidant study showed that extraction of acetic ether,n-butanol and water of the flavonoids had activity of scavenging DPPH·,whose IC_(50)value were 6.52,9.04,6.87 ug/mL,respectively.The inhibitory rate to O_2~-·of extraction of acetic ether,n-butanol and water whose concentration were 100μg/mL were 14.43% ,27.83% and 23.21%, respectively.The inhibitory rate to ·OH of extraction of acetic ether,n-butanol and water whose concentration are 500ug/mL were 23.74%、43.51% ,32.75 %.